TY - JOUR
T1 - The effect of ammonium chloride and tunicamycin on the glycoprotein content and infectivity of herpes simplex virus type 1
AU - Kousoulas, Konstantin G.
AU - Bzik, David J.
AU - Deluca, Neal
AU - Person, Stanley
PY - 1983/3
Y1 - 1983/3
N2 - Infectious virions of MP, a syncytial strain of herpes simplex virus type 1, are formed in the presence of 50 mM NH4Cl. Underglycosylated virion glycoproteins are synthesized in infected cells and are incorporated into virions in the presence of the same concentration of NH4Cl. We conclude that fully glycosylated glycoproteins are not required for viral infectivity. Virus particles, deficient in glycosylated glycoproteins, are assembled in the presence of tunicamycin but they are not infectious. The decrease in infectivity could be due to the decreased amount of the gB or possibly other peptides and/or to the lack of the high-mannose saccharides of precursor glycoproteins.
AB - Infectious virions of MP, a syncytial strain of herpes simplex virus type 1, are formed in the presence of 50 mM NH4Cl. Underglycosylated virion glycoproteins are synthesized in infected cells and are incorporated into virions in the presence of the same concentration of NH4Cl. We conclude that fully glycosylated glycoproteins are not required for viral infectivity. Virus particles, deficient in glycosylated glycoproteins, are assembled in the presence of tunicamycin but they are not infectious. The decrease in infectivity could be due to the decreased amount of the gB or possibly other peptides and/or to the lack of the high-mannose saccharides of precursor glycoproteins.
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U2 - 10.1016/0042-6822(83)90217-9
DO - 10.1016/0042-6822(83)90217-9
M3 - Article
C2 - 6301148
AN - SCOPUS:0020535197
VL - 125
SP - 468
EP - 474
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -