The effect of aging on the seminiferous epithelium of the brown Norway rat

Research output: Contribution to journalArticle

Abstract

Aging of the mammalian testis is often accompanied by loss of germ cells and, as a result, decreased daily sperm production. It is currently unknown whether cell loss is the result of aging-related changes in germ cells or whether there are also aging-related changes in the Sertoli cells that normally support germ development and differentiation. To begin to compare the effects of age on germ cells and on Sertoli cells, we examined brown Norway rats of 6, 12, 18, 21, and 24 months of age for the frequency of seminiferous tubule regression and total testis contents of transcripts for three Sertoli cell products: SGP-2, transferrin, and cyclic protein-2 (CP- 2)/cathepsin L. Histological analysis revealed no changes in the seminiferous epithelium from 6 to 12 months of age. However, from 12 to 24 months of age, the percentage of normal tubules gradually decreased from 95% to 15% of the total while the percentage of fully regressed tubules (containing no germ cells per tubule cross section) increased from 0% to 78%. In our analysis of specific Sertoli cell transcripts, we noted no change in total testis content of SGP-2 mRNA from 6 to 24 months. However, total testis content of transferrin mRNA was unchanged from 6 to 18 months, but increased by 24 months to 368% of the content of a 6-month-old testis. In contrast, total testis content of CP-2/cathepsin L mRNA was unchanged from 6 to 12 months, but decreased by 24 months to 58% of the content of a 6-month-old testis. We conclude that aging-related loss of spermatogenic cells in the brown Norway rat is accompanied by specific changes in expression of particular Sertoli cell transcripts.

Original languageEnglish (US)
Pages (from-to)110-117
Number of pages8
JournalJournal of Andrology
Volume14
Issue number2
StatePublished - 1993

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Seminiferous Epithelium
Testis
Sertoli Cells
Germ Cells
Cathepsin L
Transferrin
Messenger RNA
Seminiferous Tubules
Spermatozoa

Keywords

  • Aging
  • gene expression
  • rat
  • Sertoli cells
  • spermatogenesis

ASJC Scopus subject areas

  • Endocrinology

Cite this

The effect of aging on the seminiferous epithelium of the brown Norway rat. / Wright, William W; Fiore, C.; Zirkin, Barry R.

In: Journal of Andrology, Vol. 14, No. 2, 1993, p. 110-117.

Research output: Contribution to journalArticle

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