The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines

J. Pessoa de Souza Filho, Z. M.S. Correa, J. C. Marshall, E. Anteka, A. B. Coutinho, M. C. Martins, M. N. Burnier

Research output: Contribution to journalArticle

Abstract

Purpose: To examine the effect of nepafenac, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation rate of two human retinoblastoma (Rb)cell lines. Methods: Two human Rb cell lines (WERI-RB and Y79) were cultured. COX-2 expression in these cell lines was verified by imunocytochemical analysis of cytospin sections and Western blotting. An MTT-based proliferation assay was used to compare Rb cell growth with and without amfenac, the active metabolite of nepafenac. The averaged results per condition were recorded. The Student's t-test was used to compare results from the cells cultured with and without amfenac. Result: The Y79 cell line showed a higher proliferative rate than the WERI-RB cell line. Both cell lines were negative for COX-2 expression by immunocytochemical analysis; however, both cell lines were positive for COX-2 expression by Western blot. When amfenac was added to both of the cell lines, a statistically significant reduction in proliferation was observed in both cell lines. The two Rb cell lines were positive for COX-2 only in the Western blot, indicating that they probably express low levels of COX-2, which was undetectable by immucytochemical analysis. Conclusion: The selective, anti-COX-2 molecule amfenac inhibited proliferation of both tested Rb cell lines. Further trials should be undertaken to study the effect of selective COX-2 inhibitors on Rb.

Original languageEnglish (US)
Pages (from-to)598-601
Number of pages4
JournalEye
Volume20
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

Keywords

  • COX-2
  • Cell lines
  • Immunocytochemistry
  • Nepafenac
  • Retinoblastoma

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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    de Souza Filho, J. P., Correa, Z. M. S., Marshall, J. C., Anteka, E., Coutinho, A. B., Martins, M. C., & Burnier, M. N. (2006). The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines. Eye, 20(5), 598-601. https://doi.org/10.1038/sj.eye.6701938