The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek, Michael T. Forman, Isabella Martin, Ravit Boger, Alexandra Valsamakis

Research output: Contribution to journalArticle

Abstract

A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

Original languageEnglish (US)
Pages (from-to)40-43
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume89
Issue number1
DOIs
StatePublished - Sep 1 2017

Fingerprint

Adenoviridae Infections
Real-Time Polymerase Chain Reaction
Nucleotides
Fluorescence
Bone Marrow
Transplants

Keywords

  • Aberrant real-time PCR
  • Adenovirus
  • Reduced fluorescence

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection. / Gniadek, Thomas J.; Forman, Michael T.; Martin, Isabella; Boger, Ravit; Valsamakis, Alexandra.

In: Diagnostic Microbiology and Infectious Disease, Vol. 89, No. 1, 01.09.2017, p. 40-43.

Research output: Contribution to journalArticle

Gniadek, Thomas J. ; Forman, Michael T. ; Martin, Isabella ; Boger, Ravit ; Valsamakis, Alexandra. / The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection. In: Diagnostic Microbiology and Infectious Disease. 2017 ; Vol. 89, No. 1. pp. 40-43.
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