The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek; Michael T. Forman; Isabella Martin; Ravit Boger; Alexandra Valsamakis

doi:10.1016/j.diagmicrobio.2017.05.013

The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek, Michael T. Forman, Isabella Martin, Ravit Boger, Alexandra Valsamakis

School of Medicine

Research output: Contribution to journal › Article

Abstract

A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

Original language	English (US)
Pages (from-to)	40-43
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	89
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2017.05.013
State	Published - Sep 1 2017

Keywords

Aberrant real-time PCR
Adenovirus
Reduced fluorescence

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2017.05.013

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Gniadek, T. J., Forman, M. T., Martin, I., Boger, R., & Valsamakis, A. (2017). The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection. Diagnostic Microbiology and Infectious Disease, 89(1), 40-43. https://doi.org/10.1016/j.diagmicrobio.2017.05.013

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