The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek, Michael T. Forman, Isabella Martin, Ravit Boger, Alexandra Valsamakis

Research output: Contribution to journalArticle

Abstract

A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

Original languageEnglish (US)
Pages (from-to)40-43
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume89
Issue number1
DOIs
StatePublished - Sep 1 2017

Keywords

  • Aberrant real-time PCR
  • Adenovirus
  • Reduced fluorescence

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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