The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek; Michael T. Forman; Isabella Martin; Ravit Arav-Boger; Alexandra Valsamakis

doi:10.1016/j.diagmicrobio.2017.05.013

The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek, Michael T. Forman, Isabella Martin, Ravit Arav-Boger, Alexandra Valsamakis

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

Original language	English (US)
Pages (from-to)	40-43
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	89
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2017.05.013
State	Published - Sep 2017

Keywords

Aberrant real-time PCR
Adenovirus
Reduced fluorescence

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2017.05.013

Cite this

@article{545e69efed784946a7b16d75a37cd0c6,

title = "The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection",

abstract = "A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.",

keywords = "Aberrant real-time PCR, Adenovirus, Reduced fluorescence",

author = "Gniadek, {Thomas J.} and Forman, {Michael T.} and Isabella Martin and Ravit Arav-Boger and Alexandra Valsamakis",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

month = sep,

doi = "10.1016/j.diagmicrobio.2017.05.013",

language = "English (US)",

volume = "89",

pages = "40--43",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

AU - Gniadek, Thomas J.

AU - Forman, Michael T.

AU - Martin, Isabella

AU - Arav-Boger, Ravit

AU - Valsamakis, Alexandra

PY - 2017/9

Y1 - 2017/9

N2 - A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

AB - A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

KW - Aberrant real-time PCR

KW - Adenovirus

KW - Reduced fluorescence

UR - http://www.scopus.com/inward/record.url?scp=85020783915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020783915&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2017.05.013

DO - 10.1016/j.diagmicrobio.2017.05.013

M3 - Article

C2 - 28629877

AN - SCOPUS:85020783915

VL - 89

SP - 40

EP - 43

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 1

ER -

The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this