Abstract
A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 40-43 |
| Number of pages | 4 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Volume | 89 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 1 2017 |
Fingerprint
Keywords
- Aberrant real-time PCR
- Adenovirus
- Reduced fluorescence
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
Cite this
The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection. / Gniadek, Thomas J.; Forman, Michael T.; Martin, Isabella; Boger, Ravit; Valsamakis, Alexandra.
In: Diagnostic Microbiology and Infectious Disease, Vol. 89, No. 1, 01.09.2017, p. 40-43.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection
AU - Gniadek, Thomas J.
AU - Forman, Michael T.
AU - Martin, Isabella
AU - Boger, Ravit
AU - Valsamakis, Alexandra
PY - 2017/9/1
Y1 - 2017/9/1
N2 - A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.
AB - A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.
KW - Aberrant real-time PCR
KW - Adenovirus
KW - Reduced fluorescence
UR - http://www.scopus.com/inward/record.url?scp=85020783915&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85020783915&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2017.05.013
DO - 10.1016/j.diagmicrobio.2017.05.013
M3 - Article
C2 - 28629877
AN - SCOPUS:85020783915
VL - 89
SP - 40
EP - 43
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 1
ER -