The E Block motif is associated with Legionella pneumophila translocated substrates

Li Huang, Dana Boyd, Whitney M. Amyot, Andrew D. Hempstead, Zhao Qing Luo, Tamara J. O'Connor, Cui Chen, Matthias Machner, Timothy Montminy, Ralph R. Isberg

Research output: Contribution to journalArticle

Abstract

Legionella pneumophila promotes intracellular growth by moving bacterial proteins across membranes via the Icm/Dot system. A strategy was devised to identify large numbers of Icm/Dot translocated proteins, and the resulting pool was used to identify common motifs that operate as recognition signals. The 3' end of the sidC gene, which encodes a known translocated substrate, was replaced with DNA encoding 200 codons from the 3' end of 442 potential substrate-encoding genes. The resulting hybrid proteins were then tested in a high throughput assay, in which translocated SidC antigen was detected by indirect immunofluorescence. Among translocated substrates, regions of 6-8 residues called E Blocks were identified that were rich in glutamates. Analysis of SidM/DrrA revealed that loss of three Glu residues, arrayed in a triangle on an α-helical surface, totally eliminated translocation of a reporter protein. Based on this result, a second strategy was employed to identify Icm/Dot substrates having carboxyl terminal glutamates. From the fusion assay and the bioinformatic queries, carboxyl terminal sequences from 49 previously unidentified proteins were shown to promote translocation into target cells. These studies indicate that by analysing subsets of translocated substrates, patterns can be found that allow predictions of important motifs recognized by Icm/Dot.

Original languageEnglish (US)
Pages (from-to)227-245
Number of pages19
JournalCellular Microbiology
Volume13
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Fingerprint

Legionella pneumophila
Glutamates
Proteins
Bacterial Proteins
Indirect Fluorescent Antibody Technique
Computational Biology
Codon
Genes
Antigens
Membranes
DNA
Growth

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

The E Block motif is associated with Legionella pneumophila translocated substrates. / Huang, Li; Boyd, Dana; Amyot, Whitney M.; Hempstead, Andrew D.; Luo, Zhao Qing; O'Connor, Tamara J.; Chen, Cui; Machner, Matthias; Montminy, Timothy; Isberg, Ralph R.

In: Cellular Microbiology, Vol. 13, No. 2, 02.2011, p. 227-245.

Research output: Contribution to journalArticle

Huang, L, Boyd, D, Amyot, WM, Hempstead, AD, Luo, ZQ, O'Connor, TJ, Chen, C, Machner, M, Montminy, T & Isberg, RR 2011, 'The E Block motif is associated with Legionella pneumophila translocated substrates', Cellular Microbiology, vol. 13, no. 2, pp. 227-245. https://doi.org/10.1111/j.1462-5822.2010.01531.x
Huang, Li ; Boyd, Dana ; Amyot, Whitney M. ; Hempstead, Andrew D. ; Luo, Zhao Qing ; O'Connor, Tamara J. ; Chen, Cui ; Machner, Matthias ; Montminy, Timothy ; Isberg, Ralph R. / The E Block motif is associated with Legionella pneumophila translocated substrates. In: Cellular Microbiology. 2011 ; Vol. 13, No. 2. pp. 227-245.
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