The Dynamic Distribution of CARD11 at the Immunological Synapse Is Regulated by the Inhibitory Kinesin GAKIN

Rebecca L. Lamason, Abraham Kupfer, Joel L. Pomerantz

Research output: Contribution to journalArticle

Abstract

T cell receptor (TCR) signaling to NF-κB is required for antigen-induced T cell activation. We conducted an expression-cloning screen for modifiers of CARD11, a critical adaptor in antigen receptor signaling, and identified the kinesin-3 family member GAKIN as a CARD11 inhibitor. GAKIN negatively regulates TCR signaling to NF-κB, associates with CARD11 in a signal-dependent manner and can compete with the required signaling protein, Bcl10, for association. In addition, GAKIN dynamically localizes to the immunological synapse and regulates the redistribution of CARD11 from the central region of the synapse to a distal region. We propose that CARD11 scaffold function and occupancy at the center of the synapse are negatively regulated by GAKIN to tune the output of antigen-receptor signaling.

Original languageEnglish (US)
Pages (from-to)798-809
Number of pages12
JournalMolecular cell
Volume40
Issue number5
DOIs
StatePublished - Dec 10 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The Dynamic Distribution of CARD11 at the Immunological Synapse Is Regulated by the Inhibitory Kinesin GAKIN'. Together they form a unique fingerprint.

  • Cite this