The Drosophila Smad cofactor Schnurri engages in redundant and synergistic interactions with multiple corepressors

Yi Cai, Allen Laughon

Research output: Contribution to journalArticlepeer-review

Abstract

In Drosophila a large zinc finger protein, Schnurri, functions as a Smad cofactor required for repression of brinker and other negative targets in response to signaling by the transforming growth factor β ligand, Decapentaplegic. Schnurri binds to the silencer-bound Smads through a cluster of zinc fingers located near its carboxy-terminus and silences via a separate repression domain adjacent to this zinc-finger cluster. Here we show that this repression domain functions through interaction with two corepressors, dCtBP and dSin3A, and that either interaction is sufficient for repression. We also report that Schnurri contains additional repression domains that function through interaction with dCtBP, Groucho, dSin3A and SMRTER. By testing for the ability to rescue a shn RNAi phenotype we provide evidence that these diverse repression domains are both cooperative and partially redundant. In addition we find that Shn harbors a region capable of transcriptional activation, consistent with evidence that Schnurri can function as an activator as well as a repressor.

Original languageEnglish (US)
Pages (from-to)232-245
Number of pages14
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1789
Issue number3
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

Keywords

  • Corepressor
  • CtBP
  • Groucho
  • SMRTER
  • Schnurri
  • Sin3A

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

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