The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation

Haifan Lin, Lin Yue, Allan C. Spradling

Research output: Contribution to journalArticle

Abstract

Oogenesis in Drosophila takes place within germline cysts that support polarized transport through ring canals interconnecting their 15 nurse cells and single oocyte. Developing cystocytes are spanned by a large cytoplasmic structure known as the fusome that has been postulated to help form ring canals and determine the pattern of nurse cell-oocyte interconnections. We identified the adducin-like hts product and α-spectrin as molecular components of fusomes, discovered a related structure in germline stem cells and documented regular associations between fusomes and cystocyte centrosomes. hts mutations completely eliminated fusomes, causing abnormal cysts containing a reduced number of cells to form. Our results imply that Drosophila fusomes are required for ovarian cyst formation and suggest that membrane skeletal proteins regulate cystocyte divisions.

Original languageEnglish (US)
Pages (from-to)947-956
Number of pages10
JournalDevelopment
Volume120
Issue number4
StatePublished - Apr 1994
Externally publishedYes

Fingerprint

Organelles
Drosophila
Oocytes
Cysts
Membrane Proteins
Nurses
Cytoplasmic Structures
Spectrin
Oogenesis
Centrosome
Ovarian Cysts
Stem Cells
Cell Count
Mutation
adducin

Keywords

  • Cyst formation
  • Drosophila
  • Fusome
  • Germline
  • Membrane skeletal protein
  • Oocyte

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation. / Lin, Haifan; Yue, Lin; Spradling, Allan C.

In: Development, Vol. 120, No. 4, 04.1994, p. 947-956.

Research output: Contribution to journalArticle

Lin, Haifan ; Yue, Lin ; Spradling, Allan C. / The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation. In: Development. 1994 ; Vol. 120, No. 4. pp. 947-956.
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