The Drosophila couch potato gene: An essential gene required for normal adult behavior

H. J. Bellen, H. Vaessin, E. Bier, Alex L Kolodkin, D. D'Evelyn, S. Kooyer, Nung Jan Yuh Nung Jan

Research output: Contribution to journalArticle

Abstract

Through enhancer detection screens we have isolated 14 insertions in an essential gene that is expressed in embryonic sensory mother cells (SMC), in most cells of the mature embryonic peripheral nervous system (PNS), and in glial cells of the PNS and the central nervous system (CNS). Embryos homozygote for amorphic alleles die, but show no obvious defects in their cuticle, PNS or CNS. The gene has been named couch potato (cpo) because several insertional alleles alter adult behavior. Homozygous hypomorphic cpo flies recover slowly from ether anaesthesia, show aberrant flight behavior, fail to move toward light and do not exhibit normal negative geotactic behavior. However, the flies are able to groom and walk, and some are able to fly when prodded, indicating that not all processes required for behavior are severely affected. A molecular analysis shows that the 14 insertions are confined to a few hundred nucleotides which probably contain key regulatory sequences of the gene. The orientation of these insertions and their position within this DNA fragment play an important role in the couch potato phenotype. In situ hybridization to whole mount embryos suggest that some insertions affect the levels of transcription of cpo in most cells in which it is expressed.

Original languageEnglish (US)
Pages (from-to)365-375
Number of pages11
JournalGenetics
Volume131
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Essential Genes
Drosophila
Peripheral Nervous System
Diptera
Genes
Embryonic Structures
Central Nervous System
Alleles
Homozygote
Regulator Genes
Neuroglia
Ether
In Situ Hybridization
Stem Cells
Anesthesia
Nucleotides
Phenotype
Light
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Bellen, H. J., Vaessin, H., Bier, E., Kolodkin, A. L., D'Evelyn, D., Kooyer, S., & Yuh Nung Jan, N. J. (1992). The Drosophila couch potato gene: An essential gene required for normal adult behavior. Genetics, 131(2), 365-375.

The Drosophila couch potato gene : An essential gene required for normal adult behavior. / Bellen, H. J.; Vaessin, H.; Bier, E.; Kolodkin, Alex L; D'Evelyn, D.; Kooyer, S.; Yuh Nung Jan, Nung Jan.

In: Genetics, Vol. 131, No. 2, 1992, p. 365-375.

Research output: Contribution to journalArticle

Bellen, HJ, Vaessin, H, Bier, E, Kolodkin, AL, D'Evelyn, D, Kooyer, S & Yuh Nung Jan, NJ 1992, 'The Drosophila couch potato gene: An essential gene required for normal adult behavior', Genetics, vol. 131, no. 2, pp. 365-375.
Bellen HJ, Vaessin H, Bier E, Kolodkin AL, D'Evelyn D, Kooyer S et al. The Drosophila couch potato gene: An essential gene required for normal adult behavior. Genetics. 1992;131(2):365-375.
Bellen, H. J. ; Vaessin, H. ; Bier, E. ; Kolodkin, Alex L ; D'Evelyn, D. ; Kooyer, S. ; Yuh Nung Jan, Nung Jan. / The Drosophila couch potato gene : An essential gene required for normal adult behavior. In: Genetics. 1992 ; Vol. 131, No. 2. pp. 365-375.
@article{1487a4e1b6594512bfdc8313b3b5afa2,
title = "The Drosophila couch potato gene: An essential gene required for normal adult behavior",
abstract = "Through enhancer detection screens we have isolated 14 insertions in an essential gene that is expressed in embryonic sensory mother cells (SMC), in most cells of the mature embryonic peripheral nervous system (PNS), and in glial cells of the PNS and the central nervous system (CNS). Embryos homozygote for amorphic alleles die, but show no obvious defects in their cuticle, PNS or CNS. The gene has been named couch potato (cpo) because several insertional alleles alter adult behavior. Homozygous hypomorphic cpo flies recover slowly from ether anaesthesia, show aberrant flight behavior, fail to move toward light and do not exhibit normal negative geotactic behavior. However, the flies are able to groom and walk, and some are able to fly when prodded, indicating that not all processes required for behavior are severely affected. A molecular analysis shows that the 14 insertions are confined to a few hundred nucleotides which probably contain key regulatory sequences of the gene. The orientation of these insertions and their position within this DNA fragment play an important role in the couch potato phenotype. In situ hybridization to whole mount embryos suggest that some insertions affect the levels of transcription of cpo in most cells in which it is expressed.",
author = "Bellen, {H. J.} and H. Vaessin and E. Bier and Kolodkin, {Alex L} and D. D'Evelyn and S. Kooyer and {Yuh Nung Jan}, {Nung Jan}",
year = "1992",
language = "English (US)",
volume = "131",
pages = "365--375",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "2",

}

TY - JOUR

T1 - The Drosophila couch potato gene

T2 - An essential gene required for normal adult behavior

AU - Bellen, H. J.

AU - Vaessin, H.

AU - Bier, E.

AU - Kolodkin, Alex L

AU - D'Evelyn, D.

AU - Kooyer, S.

AU - Yuh Nung Jan, Nung Jan

PY - 1992

Y1 - 1992

N2 - Through enhancer detection screens we have isolated 14 insertions in an essential gene that is expressed in embryonic sensory mother cells (SMC), in most cells of the mature embryonic peripheral nervous system (PNS), and in glial cells of the PNS and the central nervous system (CNS). Embryos homozygote for amorphic alleles die, but show no obvious defects in their cuticle, PNS or CNS. The gene has been named couch potato (cpo) because several insertional alleles alter adult behavior. Homozygous hypomorphic cpo flies recover slowly from ether anaesthesia, show aberrant flight behavior, fail to move toward light and do not exhibit normal negative geotactic behavior. However, the flies are able to groom and walk, and some are able to fly when prodded, indicating that not all processes required for behavior are severely affected. A molecular analysis shows that the 14 insertions are confined to a few hundred nucleotides which probably contain key regulatory sequences of the gene. The orientation of these insertions and their position within this DNA fragment play an important role in the couch potato phenotype. In situ hybridization to whole mount embryos suggest that some insertions affect the levels of transcription of cpo in most cells in which it is expressed.

AB - Through enhancer detection screens we have isolated 14 insertions in an essential gene that is expressed in embryonic sensory mother cells (SMC), in most cells of the mature embryonic peripheral nervous system (PNS), and in glial cells of the PNS and the central nervous system (CNS). Embryos homozygote for amorphic alleles die, but show no obvious defects in their cuticle, PNS or CNS. The gene has been named couch potato (cpo) because several insertional alleles alter adult behavior. Homozygous hypomorphic cpo flies recover slowly from ether anaesthesia, show aberrant flight behavior, fail to move toward light and do not exhibit normal negative geotactic behavior. However, the flies are able to groom and walk, and some are able to fly when prodded, indicating that not all processes required for behavior are severely affected. A molecular analysis shows that the 14 insertions are confined to a few hundred nucleotides which probably contain key regulatory sequences of the gene. The orientation of these insertions and their position within this DNA fragment play an important role in the couch potato phenotype. In situ hybridization to whole mount embryos suggest that some insertions affect the levels of transcription of cpo in most cells in which it is expressed.

UR - http://www.scopus.com/inward/record.url?scp=0026734149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026734149&partnerID=8YFLogxK

M3 - Article

C2 - 1644278

AN - SCOPUS:0026734149

VL - 131

SP - 365

EP - 375

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 2

ER -