The DPL1 gene is involved in mediating the response to nutrient deprivation in saccharomyces cerevisiae

Danielle Gottlieb, Warren Heideman, Julie D. Saba

Research output: Contribution to journalArticlepeer-review

Abstract

Sphingosine-1-phosphate is a sphingolipid metabolite involved, in the regulation of cell proliferation in mammalian cells. The major route of sphingosine-1-phosphate degradation is through cleavage at the C2-3 bond by sphingosine phosphate lyase. The recent identification of the first dihydrosphingosine/sphingosine phosphate lyase gene in Saccharomyces cerevisiae establishes that phosphorylated sphingoid base metabolism is conserved throughout evolution. The dpl1Δ deletion mutant, which accumulates endogenous phosphorylated sphingoid bases, exhibits unregulated proliferation upon approach to stationary phase. The increased proliferation rate during respiratory growth was associated with failure to appropriately recruit cells into the G1 phase of the cell cycle. Several genes were found to be overexpressed or prematurely expressed during nutrient deprivation in the dpl1Δ strain, including glucose-repressible genes and G1 cyclins. These studies implicate a role for DPL1 and phosphorylated sphingoid bases in the regulation of global responses to nutrient deprivation in yeast.

Original languageEnglish (US)
Pages (from-to)66-71
Number of pages6
JournalMolecular Cell Biology Research Communications
Volume1
Issue number1
DOIs
StatePublished - Apr 1999
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology

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