The DNA puff gene BhC4-1 of Bradysia hygida is specifically transcribed in early prepupal salivary glands of Drosophila melanogaster

Nadia Monesi, Marcelo Jacobs-Lorena, M. L. Paçó-Larson

Research output: Contribution to journalArticle

Abstract

The BhC4-1 gene of the sciarid Bradysia hygida is located at DNA puff C4 and is amplified and actively transcribed in the salivary gland at the end of the last larval instar. We show here that a 3.6 kb fragment from the upstream region of the BhC4-1 gene is able to drive transcription in transgenic Drosophila specifically in prepupal salivary gland in a temporally regulated manner. The mRNA is present in maximal amounts in prepupae +3 h; in prepupae +9 h the levels of BhC4-1 mRNA decline, and it is no longer detected in pupae +24 h. Taken together these results suggest that most, if not all, of the key promoter regulatory elements were included in the DNA fragments employed to transform Drosophila. Moreover, strong expression of the transgenes implies conservation of the regulatory elements involved, since Drosophila transcription factors appear to recognize B. hygida regulatory DNA sequences. Quantitative Southern blot hybridization indicates that the sequences from DNA puff C4 are not amplified at detectable levels in salivary glands of transgenic prepupae when the BhC4-1 gene is transcribed. Transcription of a DNA puff in the absence of amplification indicates that the induction of these processes involves distinct mechanisms.

Original languageEnglish (US)
Pages (from-to)559-569
Number of pages11
JournalChromosoma
Volume107
Issue number8
DOIs
StatePublished - 1998
Externally publishedYes

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Salivary Glands
Drosophila melanogaster
Drosophila
DNA
Genes
Pupa
Messenger RNA
Southern Blotting
Transgenes
Transcription Factors

ASJC Scopus subject areas

  • Genetics

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The DNA puff gene BhC4-1 of Bradysia hygida is specifically transcribed in early prepupal salivary glands of Drosophila melanogaster. / Monesi, Nadia; Jacobs-Lorena, Marcelo; Paçó-Larson, M. L.

In: Chromosoma, Vol. 107, No. 8, 1998, p. 559-569.

Research output: Contribution to journalArticle

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