The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: Cell-free Ty1 transposition

Daniel J. Eichinger, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

Yeast Ty1 elements are retrotransposons that transpose via an RNA intermediate found in a virus-like particle (Ty-VLP). A Ty-encoded reverse transcriptase activity found inside the particles is capable of giving rise to full-length reverse transcripts. The predominant form of these reverse transcripts is a full-length linear duplex DNA. We háve developed a cell-free system for transposition of Ty1 DNA molecules into a bacteriophage λ target. Purified Ty-VLPs and target DNA are the only macromolecular components required for the transposition reaction. A TYB-encoded protein, p90-TYB, contains amino acid sequences that are similar to those of retroviral integrase proteins. Mutations in the integrase coding region abolish transposition both in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)955-966
Number of pages12
JournalCell
Volume54
Issue number7
DOIs
StatePublished - Sep 23 1988

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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