The distribution of glutamate receptors in cultured rat hippocampal neurons: Postsynaptic clustering of AMPA selective subunits

Ann Marie Craig, Craig D. Blackstone, Richard L Huganir, Gary Banker

Research output: Contribution to journalArticle

Abstract

The distribution of several glutamate receptor subunits was investigated in cultured rat hippocampal neurons by in situ hybridization and immunocytochemistry. The AMPA/kainate-selective receptors GIuR1-6 exhibited two patterns of mRNA expression: most neurons expressed GluR1, R2, and R6, whereas only about 20% expressed significant levels of GIuR3, R4, and R5. By immunocytochemistry, the metabotropic glutamate receptor mGluR1α was detectable only in a subpopulation of GABAergic interneurons. GluR1 and GIuR2/3 segregated to the somatodendritic domain within the first week in culture, even in the absence of synaptogenesis. Glutamate receptor-enriched spines developed later and were present only on presumptive pyramidal cells, not on GABAergic interneurons. Clusters of GIuR1 and GluR2/3 completely colocalized and were restricted to a subset of postsynaptic sites. Thus, glutamate receptor subunits exhibit both a cell type-specific expression and a selective subcellular localization.

Original languageEnglish (US)
Pages (from-to)1055-1068
Number of pages14
JournalNeuron
Volume10
Issue number6
DOIs
StatePublished - 1993

Fingerprint

alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Glutamate Receptors
Cluster Analysis
Interneurons
Neurons
Immunohistochemistry
Kainic Acid Receptors
Metabotropic Glutamate Receptors
Pyramidal Cells
In Situ Hybridization
Spine
Messenger RNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The distribution of glutamate receptors in cultured rat hippocampal neurons : Postsynaptic clustering of AMPA selective subunits. / Craig, Ann Marie; Blackstone, Craig D.; Huganir, Richard L; Banker, Gary.

In: Neuron, Vol. 10, No. 6, 1993, p. 1055-1068.

Research output: Contribution to journalArticle

@article{312583ac93544e26b00f0fd9d3ede8d5,
title = "The distribution of glutamate receptors in cultured rat hippocampal neurons: Postsynaptic clustering of AMPA selective subunits",
abstract = "The distribution of several glutamate receptor subunits was investigated in cultured rat hippocampal neurons by in situ hybridization and immunocytochemistry. The AMPA/kainate-selective receptors GIuR1-6 exhibited two patterns of mRNA expression: most neurons expressed GluR1, R2, and R6, whereas only about 20{\%} expressed significant levels of GIuR3, R4, and R5. By immunocytochemistry, the metabotropic glutamate receptor mGluR1α was detectable only in a subpopulation of GABAergic interneurons. GluR1 and GIuR2/3 segregated to the somatodendritic domain within the first week in culture, even in the absence of synaptogenesis. Glutamate receptor-enriched spines developed later and were present only on presumptive pyramidal cells, not on GABAergic interneurons. Clusters of GIuR1 and GluR2/3 completely colocalized and were restricted to a subset of postsynaptic sites. Thus, glutamate receptor subunits exhibit both a cell type-specific expression and a selective subcellular localization.",
author = "Craig, {Ann Marie} and Blackstone, {Craig D.} and Huganir, {Richard L} and Gary Banker",
year = "1993",
doi = "10.1016/0896-6273(93)90054-U",
language = "English (US)",
volume = "10",
pages = "1055--1068",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - The distribution of glutamate receptors in cultured rat hippocampal neurons

T2 - Postsynaptic clustering of AMPA selective subunits

AU - Craig, Ann Marie

AU - Blackstone, Craig D.

AU - Huganir, Richard L

AU - Banker, Gary

PY - 1993

Y1 - 1993

N2 - The distribution of several glutamate receptor subunits was investigated in cultured rat hippocampal neurons by in situ hybridization and immunocytochemistry. The AMPA/kainate-selective receptors GIuR1-6 exhibited two patterns of mRNA expression: most neurons expressed GluR1, R2, and R6, whereas only about 20% expressed significant levels of GIuR3, R4, and R5. By immunocytochemistry, the metabotropic glutamate receptor mGluR1α was detectable only in a subpopulation of GABAergic interneurons. GluR1 and GIuR2/3 segregated to the somatodendritic domain within the first week in culture, even in the absence of synaptogenesis. Glutamate receptor-enriched spines developed later and were present only on presumptive pyramidal cells, not on GABAergic interneurons. Clusters of GIuR1 and GluR2/3 completely colocalized and were restricted to a subset of postsynaptic sites. Thus, glutamate receptor subunits exhibit both a cell type-specific expression and a selective subcellular localization.

AB - The distribution of several glutamate receptor subunits was investigated in cultured rat hippocampal neurons by in situ hybridization and immunocytochemistry. The AMPA/kainate-selective receptors GIuR1-6 exhibited two patterns of mRNA expression: most neurons expressed GluR1, R2, and R6, whereas only about 20% expressed significant levels of GIuR3, R4, and R5. By immunocytochemistry, the metabotropic glutamate receptor mGluR1α was detectable only in a subpopulation of GABAergic interneurons. GluR1 and GIuR2/3 segregated to the somatodendritic domain within the first week in culture, even in the absence of synaptogenesis. Glutamate receptor-enriched spines developed later and were present only on presumptive pyramidal cells, not on GABAergic interneurons. Clusters of GIuR1 and GluR2/3 completely colocalized and were restricted to a subset of postsynaptic sites. Thus, glutamate receptor subunits exhibit both a cell type-specific expression and a selective subcellular localization.

UR - http://www.scopus.com/inward/record.url?scp=0027273473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027273473&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(93)90054-U

DO - 10.1016/0896-6273(93)90054-U

M3 - Article

C2 - 7686378

AN - SCOPUS:0027273473

VL - 10

SP - 1055

EP - 1068

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 6

ER -