The dissociation of evoked release of [3H]-GABA and of endogenous GABA from chick retina in vitro

Peter Campochiaro, John W. Ferkany, Joseph T. Coyle

Research output: Contribution to journalArticle

Abstract

The release of prelabeled [3H]-GABA and endogenous GABA evoked by KCl and excitatory amino acid analogs was compared in intact chick retina and retina previously lesioned by the neurotoxins, kainic acid and N-methyl-d,l-aspartic acid. Significant discrepancies were found in the results obtained by the two techniques; and while changes in endogenous GABA release after neurotoxin lesions correlated with retinal glutamate decarboxylase activity, a marker for GABAergic neurons, the release of [3H]-GABA did not. These results call into question the validity of the prelabeling technique for studying GABA release from retina.

Original languageEnglish (US)
Pages (from-to)299-305
Number of pages7
JournalExperimental eye research
Volume39
Issue number3
DOIs
StatePublished - Sep 1984

Keywords

  • GABA
  • endogenous
  • neurotransmitter
  • prelabeled
  • release
  • retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'The dissociation of evoked release of [<sup>3</sup>H]-GABA and of endogenous GABA from chick retina in vitro'. Together they form a unique fingerprint.

  • Cite this