TY - JOUR
T1 - The differential effect of scaffold composition and architecture on chondrocyte response to mechanical stimulation
AU - Appelman, Taly P.
AU - Mizrahi, Joseph
AU - Elisseeff, Jennifer H.
AU - Seliktar, Dror
N1 - Funding Information:
The authors gratefully acknowledge the financial support of the United States-Israel Binational Science Foundation (grant no. 2003184) and the joint Johns Hopkins University-Technion Program for Biomedical Sciences and Biomedical Engineering. The authors thank Ms. Liat Oss-Ronen for providing the PEG-Albumin for this study.
PY - 2009/2
Y1 - 2009/2
N2 - This study aims to explore the differential effect of scaffold composition and architecture on chondrogenic response to dynamic strain stimulation using encapsulating PEG-based hydrogels and primary bovine chondrocytes. Proteins and proteoglycans were conjugated to functionalized poly(ethylene glycol) (PEG) and immobilized in PEG hydrogels to create bio-synthetic materials to be used as scaffolds. Four different compositions were tested, including: PEG-Proteoglycan (PP), PEG-Fibrinogen (PF), PEG-Albumin (PA), and PEG only. Primary articular chondrocytes were encapsulated in the hydrogel scaffolds and subjected to 15% dynamic compressive strain stimulation at 1-Hz frequency for 28 days. Stimulation of PP, PF, PA and PEG constructs resulted in a respective increase in the unconfined true compressive modulus by 32%, 45.4%, 33.6%, and 28.2%, compared to their static controls. The PF showed a significantly larger relative increase in the modulus in comparison to all other scaffolds tested. These results support the hypothesis that mechanical stimulation and material bioactivity have a significant effect on the reported chondrocyte response. Similar trends were observed with the swelling ratio of the constructs. These findings indicate that while stimulation causes metabolic changes in chondrocytes seeded in PEG hydrogels, the matrix bioactivity has a significant role in enhancing chondrocyte mechanotransduction in encapsulating scaffolds subjected to physical deformations.
AB - This study aims to explore the differential effect of scaffold composition and architecture on chondrogenic response to dynamic strain stimulation using encapsulating PEG-based hydrogels and primary bovine chondrocytes. Proteins and proteoglycans were conjugated to functionalized poly(ethylene glycol) (PEG) and immobilized in PEG hydrogels to create bio-synthetic materials to be used as scaffolds. Four different compositions were tested, including: PEG-Proteoglycan (PP), PEG-Fibrinogen (PF), PEG-Albumin (PA), and PEG only. Primary articular chondrocytes were encapsulated in the hydrogel scaffolds and subjected to 15% dynamic compressive strain stimulation at 1-Hz frequency for 28 days. Stimulation of PP, PF, PA and PEG constructs resulted in a respective increase in the unconfined true compressive modulus by 32%, 45.4%, 33.6%, and 28.2%, compared to their static controls. The PF showed a significantly larger relative increase in the modulus in comparison to all other scaffolds tested. These results support the hypothesis that mechanical stimulation and material bioactivity have a significant effect on the reported chondrocyte response. Similar trends were observed with the swelling ratio of the constructs. These findings indicate that while stimulation causes metabolic changes in chondrocytes seeded in PEG hydrogels, the matrix bioactivity has a significant role in enhancing chondrocyte mechanotransduction in encapsulating scaffolds subjected to physical deformations.
KW - Bioreactor
KW - Cartilage tissue engineering
KW - Hydrogel
KW - Mechanotransduction
KW - Poly(ethylene glycol)
UR - http://www.scopus.com/inward/record.url?scp=56449084391&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56449084391&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2008.09.063
DO - 10.1016/j.biomaterials.2008.09.063
M3 - Article
C2 - 19000634
AN - SCOPUS:56449084391
SN - 0142-9612
VL - 30
SP - 518
EP - 525
JO - Biomaterials
JF - Biomaterials
IS - 4
ER -