The diagnostic role of claudins in serous effusions

Lilach Kleinberg, Arild Holth, Eduard Fridman, Ignat Schwartz, Ie Ming Shih, Ben Davidson

Research output: Contribution to journalArticle


We analyzed the diagnostic role of claudins in effusion cytology in 325 effusions, including 218 ovarian, 49 breast, 15 cervical or endometrial, 10 gastrointestinal, and 8 lung adenocarcinomas and 25 malignant mesotheliomas (MMs). Specimens were analyzed for claudin-1 and claudin-3 expression using immunohistochemical analysis. Ovarian and breast adenocarcinoma were further analyzed for claudin-7 expression. Claudin-1 expression was most frequent in ovarian and cervical or endometrial adenocarcinoma compared with other adenocarcinomas and MMs (P < .001). Claudin-3 expression was comparable in adenocarcinomas of different origin but was absent in MMs (P < .001). Reactive mesothelial cells rarely expressed claudins. Claudin-7 expression was higher in ovarian than in breast adenocarcinoma (P < .001). Our data suggest that expression of claudin-3 or claudin-7 is specific for adenocarcinoma and rules out the diagnosis of cells as mesothelial and that absence of claudin-1 expression excludes ovarian carcinoma as the possible origin of metastatic adenocarcinoma. Claudins may, therefore, be of diagnostic value in effusion cytology.

Original languageEnglish (US)
Pages (from-to)928-937
Number of pages10
JournalAmerican journal of clinical pathology
Issue number6
StatePublished - Jun 1 2007


  • Adenocarcinoma
  • Claudins
  • Effusions
  • Mesothelioma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Kleinberg, L., Holth, A., Fridman, E., Schwartz, I., Shih, I. M., & Davidson, B. (2007). The diagnostic role of claudins in serous effusions. American journal of clinical pathology, 127(6), 928-937.