The development of a monoclonal antibody to a human gamma crystallin

Paul Russell, J. Samuel Zigler, Venkat Reddy

Research output: Contribution to journalArticle

Abstract

A monoclonal antibody to human lens 21,000 molecular weight gamma crystallin has been produced in rat x mouse hybridoma cell lines. The antibody reacts specifically with the 24,000 molecular weight gamma crystallin and does not cross react with the 21,000 or the 19,000 gamma crystallins. The antibody reacts with protein from dog, mouse, monkey and bovine lenses but does not react with proteins from chick lens. The 24,000 molecular weight gamma protein increases from 2% of the soluble protein in the fetal lens to 14% in the 2 month old lens to 25? in the adult lens. No difference was observed in the amount of this crystallin between the nuclear and cortical regions of the adult lens. In one hypermature cataractous lens, the amount of this crystallin decreased to a value of about 5% of the total soluble protein.

Original languageEnglish (US)
Pages (from-to)1329-1335
Number of pages7
JournalCurrent Eye Research
Volume3
Issue number11
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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