The development and functions of CD4 T cells expressing a transgenic TCR specific for an MHC-I-restricted tumor antigenic epitope

Xue Han, Peiying Ye, Liqun Luo, Linghua Zheng, Yang Liu, Lieping Chen, Shengdian Wang

Research output: Contribution to journalArticle

Abstract

It has been reported that the ratio of CD4 + to CD8 + T cells has no bias in a few class I major histocompatibility complex (MHC-I)-restricted T-cell receptor (TCR)-transgenic mice specific for alloantigens or autoantigens, in which most CD4 T cells express an MHC-I-restricted TCR. In this study, we further showed that more than 50% of CD4 + T cells in MHC-I-restricted P1A tumor antigen-specific TCR (P1ATCR)-transgenic mice could specifically bind to MHC-I/P1A peptide complex. P1A peptide could stimulate the transgenic CD4 + T cells to proliferate and secrete both type 1 helper T cell and type 2 helper T cell cytokines. The activated CD4 + T cells also showed cytotoxicity against P1A-expressing tumor cells. The analysis of TCR α-chains showed that these CD4 + T cells were selected by co-expressing endogenous TCRs. Our results show that CD4 + T cells from P1ATCR transgenic mice co-expressed an MHC-I-restricted transgenic TCR and another rearranged endogenous TCRs, both of which were functional.

Original languageEnglish (US)
Pages (from-to)333-340
Number of pages8
JournalCellular and Molecular Immunology
Volume8
Issue number4
DOIs
Publication statusPublished - Jul 2011
Externally publishedYes

    Fingerprint

Keywords

  • CD4 T cells
  • MHC-I restriction
  • P1A tumor antigen
  • TCR-transgenic mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this