Abstract
It has been reported that the ratio of CD4 + to CD8 + T cells has no bias in a few class I major histocompatibility complex (MHC-I)-restricted T-cell receptor (TCR)-transgenic mice specific for alloantigens or autoantigens, in which most CD4 T cells express an MHC-I-restricted TCR. In this study, we further showed that more than 50% of CD4 + T cells in MHC-I-restricted P1A tumor antigen-specific TCR (P1ATCR)-transgenic mice could specifically bind to MHC-I/P1A peptide complex. P1A peptide could stimulate the transgenic CD4 + T cells to proliferate and secrete both type 1 helper T cell and type 2 helper T cell cytokines. The activated CD4 + T cells also showed cytotoxicity against P1A-expressing tumor cells. The analysis of TCR α-chains showed that these CD4 + T cells were selected by co-expressing endogenous TCRs. Our results show that CD4 + T cells from P1ATCR transgenic mice co-expressed an MHC-I-restricted transgenic TCR and another rearranged endogenous TCRs, both of which were functional.
Original language | English (US) |
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Pages (from-to) | 333-340 |
Number of pages | 8 |
Journal | Cellular and Molecular Immunology |
Volume | 8 |
Issue number | 4 |
DOIs | |
State | Published - Jul 2011 |
Externally published | Yes |
Keywords
- CD4 T cells
- MHC-I restriction
- P1A tumor antigen
- TCR-transgenic mice
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
- Immunology