The determination of lactic dehydrogenase with a tetrazolium salt

Marvin M. Nachlas, Stanley I. Margulies, Jerome D. Goldberg, Arnold M. Seligman

Research output: Contribution to journalArticle

Abstract

A colorimetric method for the measurement of lactic dehydrogenase activity has been described, utilizing phenazine methosulfate as an intermediate agent for electron transfer from DPNH to a tetrazolium salt (INT). The use of gelatin to keep the formazan in colloidal suspension provided the added advantage of improving electron transfer via a phenazine methosulfate gelatin complex over that obtained with soluble diaphorase or phenazine methosulfate alone. Several compounds were studied for their ability to act as intermediate electron-transfer reagents, but none were superior to phenazine methosulfate. The sensitivity of the new method is comparable to others measuring either DPNH or pyruvate; its advantages are those of brevity and simplicity. Normal human sera were found to contain from 50 to 100 units of lactic dehydrogenase. The importance of avoiding hemolysis of the blood has been re-emphasized, and the possibility has been demonstrated that a 10-15% overestimation may occur due to hemolysis even when gross hemolysis cannot be recognized.

Original languageEnglish (US)
Pages (from-to)317-326
Number of pages10
JournalAnalytical Biochemistry
Volume1
Issue number4-5
StatePublished - Dec 10 1960

Fingerprint

Methylphenazonium Methosulfate
Tetrazolium Salts
Oxidoreductases
Milk
Hemolysis
Electrons
Gelatin
Formazans
Pyruvic Acid
Suspensions
Blood
Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Nachlas, M. M., Margulies, S. I., Goldberg, J. D., & Seligman, A. M. (1960). The determination of lactic dehydrogenase with a tetrazolium salt. Analytical Biochemistry, 1(4-5), 317-326.

The determination of lactic dehydrogenase with a tetrazolium salt. / Nachlas, Marvin M.; Margulies, Stanley I.; Goldberg, Jerome D.; Seligman, Arnold M.

In: Analytical Biochemistry, Vol. 1, No. 4-5, 10.12.1960, p. 317-326.

Research output: Contribution to journalArticle

Nachlas, MM, Margulies, SI, Goldberg, JD & Seligman, AM 1960, 'The determination of lactic dehydrogenase with a tetrazolium salt', Analytical Biochemistry, vol. 1, no. 4-5, pp. 317-326.
Nachlas MM, Margulies SI, Goldberg JD, Seligman AM. The determination of lactic dehydrogenase with a tetrazolium salt. Analytical Biochemistry. 1960 Dec 10;1(4-5):317-326.
Nachlas, Marvin M. ; Margulies, Stanley I. ; Goldberg, Jerome D. ; Seligman, Arnold M. / The determination of lactic dehydrogenase with a tetrazolium salt. In: Analytical Biochemistry. 1960 ; Vol. 1, No. 4-5. pp. 317-326.
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