The design and application of genetically encodable biosensors based on fluorescent proteins

Robert H. Newman; Jin Zhang

doi:10.1007/978-1-62703-622-1-1

The design and application of genetically encodable biosensors based on fluorescent proteins

Robert H. Newman, Jin Zhang

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Pages	1-16
Number of pages	16
Volume	1071
DOIs	https://doi.org/10.1007/978-1-62703-622-1-1
State	Published - 2014

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-622-1-1

Cite this

@inbook{b01f845adba248e2a8e9ac18cf5baa54,

title = "The design and application of genetically encodable biosensors based on fluorescent proteins",

abstract = "To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.",

author = "Newman, {Robert H.} and Jin Zhang",

year = "2014",

doi = "10.1007/978-1-62703-622-1-1",

language = "English (US)",

volume = "1071",

pages = "1--16",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - The design and application of genetically encodable biosensors based on fluorescent proteins

AU - Newman, Robert H.

AU - Zhang, Jin

PY - 2014

Y1 - 2014

N2 - To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.

AB - To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.

UR - http://www.scopus.com/inward/record.url?scp=84885597346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885597346&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-622-1-1

DO - 10.1007/978-1-62703-622-1-1

M3 - Chapter

C2 - 24052376

VL - 1071

SP - 1

EP - 16

BT - Methods in Molecular Biology

ER -

The design and application of genetically encodable biosensors based on fluorescent proteins

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this