The design and application of genetically encodable biosensors based on fluorescent proteins

Robert H. Newman, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-16
Number of pages16
Volume1071
ISBN (Print)9781627036214
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1071
ISSN (Print)10643745

Keywords

  • Biosensor design
  • Cell signaling
  • Fluorescent biosensor
  • Fluorescent proteins
  • FRET
  • Live cell imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Newman, R. H., & Zhang, J. (2014). The design and application of genetically encodable biosensors based on fluorescent proteins. In Methods in Molecular Biology (Vol. 1071, pp. 1-16). (Methods in Molecular Biology; Vol. 1071). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-622-1_1