Abstract
We have shown that the 26 bp bx1 element from the regulatory region of Distal-less is capable of imposing control by the homeotic genes Ultrabithorax and abdominal-A on a general epidermal activator in Drosophila. This provides us with an assay to analyze the sequence requirements for specific repression by these Hox genes. Both the core Hox binding site, 5'- TAAT, and the adjacent EXD 5'-TGAT core site are required for repression by Ultrabithorax and abdominal-A. The Distal-less bx1 site thus fits with the model of Hox protein binding specificity based on the consensus PBX/HOX- family site TGATNNAT[g/t][g/a], where the key elements of binding specificity are proposed to lie in the two base pairs following the TGAT. A single base pair deletion in the bx1 sequence generates a site, bx1:A-mut, that on the consensus PBX/HOX model would be expected to be regulated by the Deformed Hox gene. We observed, however, that the bx1:A-mut site was regulated predominantly by Sex combs reduced, Ultrabithorax and abdominal-A. The analysis of this site indicates that the specificity of action of Hox proteins may depend not only on selective DNA binding but also on specific post-binding interactions. (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 217-226 |
| Number of pages | 10 |
| Journal | Mechanisms of Development |
| Volume | 91 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Mar 1 2000 |
| Externally published | Yes |
Fingerprint
Keywords
- Distal-less
- DNA binding protein
- Drosophila
- Extradenticle
- Gene regulation
- Grainyhead
- Homeotic gene
- Hox gene
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
The design and analysis of a homeotic response element. / White, Robert A H; Aspland, Simon; Brookman, Jenny J.; Clayton, Lesley; Sproat, Graham.
In: Mechanisms of Development, Vol. 91, No. 1-2, 01.03.2000, p. 217-226.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The design and analysis of a homeotic response element
AU - White, Robert A H
AU - Aspland, Simon
AU - Brookman, Jenny J.
AU - Clayton, Lesley
AU - Sproat, Graham
PY - 2000/3/1
Y1 - 2000/3/1
N2 - We have shown that the 26 bp bx1 element from the regulatory region of Distal-less is capable of imposing control by the homeotic genes Ultrabithorax and abdominal-A on a general epidermal activator in Drosophila. This provides us with an assay to analyze the sequence requirements for specific repression by these Hox genes. Both the core Hox binding site, 5'- TAAT, and the adjacent EXD 5'-TGAT core site are required for repression by Ultrabithorax and abdominal-A. The Distal-less bx1 site thus fits with the model of Hox protein binding specificity based on the consensus PBX/HOX- family site TGATNNAT[g/t][g/a], where the key elements of binding specificity are proposed to lie in the two base pairs following the TGAT. A single base pair deletion in the bx1 sequence generates a site, bx1:A-mut, that on the consensus PBX/HOX model would be expected to be regulated by the Deformed Hox gene. We observed, however, that the bx1:A-mut site was regulated predominantly by Sex combs reduced, Ultrabithorax and abdominal-A. The analysis of this site indicates that the specificity of action of Hox proteins may depend not only on selective DNA binding but also on specific post-binding interactions. (C) 2000 Elsevier Science Ireland Ltd.
AB - We have shown that the 26 bp bx1 element from the regulatory region of Distal-less is capable of imposing control by the homeotic genes Ultrabithorax and abdominal-A on a general epidermal activator in Drosophila. This provides us with an assay to analyze the sequence requirements for specific repression by these Hox genes. Both the core Hox binding site, 5'- TAAT, and the adjacent EXD 5'-TGAT core site are required for repression by Ultrabithorax and abdominal-A. The Distal-less bx1 site thus fits with the model of Hox protein binding specificity based on the consensus PBX/HOX- family site TGATNNAT[g/t][g/a], where the key elements of binding specificity are proposed to lie in the two base pairs following the TGAT. A single base pair deletion in the bx1 sequence generates a site, bx1:A-mut, that on the consensus PBX/HOX model would be expected to be regulated by the Deformed Hox gene. We observed, however, that the bx1:A-mut site was regulated predominantly by Sex combs reduced, Ultrabithorax and abdominal-A. The analysis of this site indicates that the specificity of action of Hox proteins may depend not only on selective DNA binding but also on specific post-binding interactions. (C) 2000 Elsevier Science Ireland Ltd.
KW - Distal-less
KW - DNA binding protein
KW - Drosophila
KW - Extradenticle
KW - Gene regulation
KW - Grainyhead
KW - Homeotic gene
KW - Hox gene
UR - http://www.scopus.com/inward/record.url?scp=0034000965&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034000965&partnerID=8YFLogxK
U2 - 10.1016/S0925-4773(99)00306-8
DO - 10.1016/S0925-4773(99)00306-8
M3 - Article
C2 - 10704846
AN - SCOPUS:0034000965
VL - 91
SP - 217
EP - 226
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 1-2
ER -