The design and analysis of a homeotic response element

Robert A H White, Simon Aspland, Jenny J. Brookman, Lesley Clayton, Graham Sproat

Research output: Contribution to journalArticle

Abstract

We have shown that the 26 bp bx1 element from the regulatory region of Distal-less is capable of imposing control by the homeotic genes Ultrabithorax and abdominal-A on a general epidermal activator in Drosophila. This provides us with an assay to analyze the sequence requirements for specific repression by these Hox genes. Both the core Hox binding site, 5'- TAAT, and the adjacent EXD 5'-TGAT core site are required for repression by Ultrabithorax and abdominal-A. The Distal-less bx1 site thus fits with the model of Hox protein binding specificity based on the consensus PBX/HOX- family site TGATNNAT[g/t][g/a], where the key elements of binding specificity are proposed to lie in the two base pairs following the TGAT. A single base pair deletion in the bx1 sequence generates a site, bx1:A-mut, that on the consensus PBX/HOX model would be expected to be regulated by the Deformed Hox gene. We observed, however, that the bx1:A-mut site was regulated predominantly by Sex combs reduced, Ultrabithorax and abdominal-A. The analysis of this site indicates that the specificity of action of Hox proteins may depend not only on selective DNA binding but also on specific post-binding interactions. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)217-226
Number of pages10
JournalMechanisms of Development
Volume91
Issue number1-2
DOIs
StatePublished - Mar 1 2000
Externally publishedYes

Fingerprint

Homeobox Genes
Response Elements
Base Pairing
Comb and Wattles
Nucleic Acid Regulatory Sequences
Protein Binding
Drosophila
Sequence Analysis
Binding Sites
DNA
Proteins

Keywords

  • Distal-less
  • DNA binding protein
  • Drosophila
  • Extradenticle
  • Gene regulation
  • Grainyhead
  • Homeotic gene
  • Hox gene

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

The design and analysis of a homeotic response element. / White, Robert A H; Aspland, Simon; Brookman, Jenny J.; Clayton, Lesley; Sproat, Graham.

In: Mechanisms of Development, Vol. 91, No. 1-2, 01.03.2000, p. 217-226.

Research output: Contribution to journalArticle

White, RAH, Aspland, S, Brookman, JJ, Clayton, L & Sproat, G 2000, 'The design and analysis of a homeotic response element', Mechanisms of Development, vol. 91, no. 1-2, pp. 217-226. https://doi.org/10.1016/S0925-4773(99)00306-8
White, Robert A H ; Aspland, Simon ; Brookman, Jenny J. ; Clayton, Lesley ; Sproat, Graham. / The design and analysis of a homeotic response element. In: Mechanisms of Development. 2000 ; Vol. 91, No. 1-2. pp. 217-226.
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