The decreased growth rate of obese strain chicken thyroid cells provides in vitro evidence for a primary target organ abnormality in chickens susceptible to autoimmune thyroiditis

Judith L. Truden; Roy S. Sundick; Seymour Levine; Noel R. Rose

doi:10.1016/0090-1229(83)90031-4

The decreased growth rate of obese strain chicken thyroid cells provides in vitro evidence for a primary target organ abnormality in chickens susceptible to autoimmune thyroiditis

Judith L. Truden, Roy S. Sundick, Seymour Levine, Noel R. Rose

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The obese (OS) strain of chickens develops severe autoimmune thyroiditis, whereas its progenitor, the Cornell (CS) strain, rarely does. To detect possible developmental abnormalities in the OS thyroid, CS and OS embryonic thyroid epithelia were cultured in vitro and compared in several assays for growth and DNA metabolism. It was found that OS thyroid cells incorporated less tritiated thymidine, reached lower saturation densities, and possessed a significantly longer doubling time (26.1 hr) than CS cells (17.6 hr). The decreased growth rate of OS thyroid cells correlated with their inability to condition their own media. These abnormalities in the embryonic development of OS thyroid cells were discussed in the context of factors predisposing to autoimmune thyroiditis.

Original language	English (US)
Pages (from-to)	294-305
Number of pages	12
Journal	Clinical Immunology and Immunopathology
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/0090-1229(83)90031-4
State	Published - Nov 1983
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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10.1016/0090-1229(83)90031-4

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The decreased growth rate of obese strain chicken thyroid cells provides in vitro evidence for a primary target organ abnormality in chickens susceptible to autoimmune thyroiditis. / Truden, Judith L.; Sundick, Roy S.; Levine, Seymour et al.
In: Clinical Immunology and Immunopathology, Vol. 29, No. 2, 11.1983, p. 294-305.

Research output: Contribution to journal › Article › peer-review

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title = "The decreased growth rate of obese strain chicken thyroid cells provides in vitro evidence for a primary target organ abnormality in chickens susceptible to autoimmune thyroiditis",

abstract = "The obese (OS) strain of chickens develops severe autoimmune thyroiditis, whereas its progenitor, the Cornell (CS) strain, rarely does. To detect possible developmental abnormalities in the OS thyroid, CS and OS embryonic thyroid epithelia were cultured in vitro and compared in several assays for growth and DNA metabolism. It was found that OS thyroid cells incorporated less tritiated thymidine, reached lower saturation densities, and possessed a significantly longer doubling time (26.1 hr) than CS cells (17.6 hr). The decreased growth rate of OS thyroid cells correlated with their inability to condition their own media. These abnormalities in the embryonic development of OS thyroid cells were discussed in the context of factors predisposing to autoimmune thyroiditis.",

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note = "Funding Information: I This work was supported by NIH Grant AM20028. z To whom reprint requests should be sent. {\textquoteright} Present address: Department of Immunology and Infectious Diseases, Johns Hopkins School of Hygiene and Public Health, 615 North Wolfe St., Baltimore, Md. 21205.",

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AU - Levine, Seymour

AU - Rose, Noel R.

N1 - Funding Information: I This work was supported by NIH Grant AM20028. z To whom reprint requests should be sent. ’ Present address: Department of Immunology and Infectious Diseases, Johns Hopkins School of Hygiene and Public Health, 615 North Wolfe St., Baltimore, Md. 21205.

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N2 - The obese (OS) strain of chickens develops severe autoimmune thyroiditis, whereas its progenitor, the Cornell (CS) strain, rarely does. To detect possible developmental abnormalities in the OS thyroid, CS and OS embryonic thyroid epithelia were cultured in vitro and compared in several assays for growth and DNA metabolism. It was found that OS thyroid cells incorporated less tritiated thymidine, reached lower saturation densities, and possessed a significantly longer doubling time (26.1 hr) than CS cells (17.6 hr). The decreased growth rate of OS thyroid cells correlated with their inability to condition their own media. These abnormalities in the embryonic development of OS thyroid cells were discussed in the context of factors predisposing to autoimmune thyroiditis.

AB - The obese (OS) strain of chickens develops severe autoimmune thyroiditis, whereas its progenitor, the Cornell (CS) strain, rarely does. To detect possible developmental abnormalities in the OS thyroid, CS and OS embryonic thyroid epithelia were cultured in vitro and compared in several assays for growth and DNA metabolism. It was found that OS thyroid cells incorporated less tritiated thymidine, reached lower saturation densities, and possessed a significantly longer doubling time (26.1 hr) than CS cells (17.6 hr). The decreased growth rate of OS thyroid cells correlated with their inability to condition their own media. These abnormalities in the embryonic development of OS thyroid cells were discussed in the context of factors predisposing to autoimmune thyroiditis.

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The decreased growth rate of obese strain chicken thyroid cells provides in vitro evidence for a primary target organ abnormality in chickens susceptible to autoimmune thyroiditis

Abstract

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