The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule

Vincent H. Ramey, Hong Wei Wang, Yuko Nakajima, Amanda Wong, Jian Liu, David Drubin, Georjana Barnes, Eva Nogales

Research output: Contribution to journalArticle

Abstract

There has been much effort in recent years aimed at understanding the molecular mechanism by which the Dam1 kinetochore complex is able to couple microtubule depolymerization to poleward movement. Both a biased diffusion and a forced walk model have been proposed, and several key functional aspects of Dam1-microtubule binding are disputed. Here, we investigate the elements involved in tubulin-Dam1 complex interactions and directly visualize Dam1 rings on microtubules in order to infer their dynamic behavior on the microtubule lattice and its likely relevance at the kinetochore. We find that the Dam1 complex has a preference for native tubulin over tubulin that is lacking its acidic C-terminal tail. Statistical mechanical analysis of images of Dam1 rings on microtubules, applied to both the distance between rings and the tilt angle of the rings with respect to the microtubule axis, supports a diffusive ring model. We also present a cryo-EM reconstruction of the Dam1 ring, likely the relevant assembly form of the complex for energy coupling during microtubule depolymerization in budding yeast. The present studies constitute a significant step forward by linking structural and biochemical observations toward a comprehensive understanding of the Dam1 complex.

Original languageEnglish (US)
Pages (from-to)457-466
Number of pages10
JournalMolecular Biology of the Cell
Volume22
Issue number4
DOIs
StatePublished - Feb 15 2011
Externally publishedYes

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Tubulin
Microtubules
Kinetochores
Saccharomycetales

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Ramey, V. H., Wang, H. W., Nakajima, Y., Wong, A., Liu, J., Drubin, D., ... Nogales, E. (2011). The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule. Molecular Biology of the Cell, 22(4), 457-466. https://doi.org/10.1091/mbc.E10-10-0841

The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule. / Ramey, Vincent H.; Wang, Hong Wei; Nakajima, Yuko; Wong, Amanda; Liu, Jian; Drubin, David; Barnes, Georjana; Nogales, Eva.

In: Molecular Biology of the Cell, Vol. 22, No. 4, 15.02.2011, p. 457-466.

Research output: Contribution to journalArticle

Ramey, VH, Wang, HW, Nakajima, Y, Wong, A, Liu, J, Drubin, D, Barnes, G & Nogales, E 2011, 'The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule', Molecular Biology of the Cell, vol. 22, no. 4, pp. 457-466. https://doi.org/10.1091/mbc.E10-10-0841
Ramey, Vincent H. ; Wang, Hong Wei ; Nakajima, Yuko ; Wong, Amanda ; Liu, Jian ; Drubin, David ; Barnes, Georjana ; Nogales, Eva. / The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 4. pp. 457-466.
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