The CP110-interacting proteins talpid3 and cep290 play overlapping and distinct roles in cilia assembly

Tetsuo Kobayashi, Sehyun Kim, Yu Chun Lin, Takanari Inoue, Brian David Dynlacht

Research output: Contribution to journalArticle

Abstract

We have identified Talpid3/KIAA0586 as a component of a CP110-containing protein complex important for centrosome and cilia function. Talpid3 assembles a ring-like structure at the extreme distal end of centrioles. Ablation of Talpid3 resulted in an aberrant distribution of centriolar satellites involved in protein trafficking to centrosomes as well as cilia assembly defects, reminiscent of loss of Cep290, another CP110-associated protein. Talpid3 depletion also led to mislocalization of Rab8a, a small GTPase thought to be essential for ciliary vesicle formation. Expression of activated Rab8a suppressed cilia assembly defects provoked by Talpid3 depletion, suggesting that Talpid3 affects cilia formation through Rab8a recruitment and/or activation. Remarkably, ultrastructural analyses showed that Talpid3 is required for centriolar satellite dispersal, which precedes the formation of mature ciliary vesicles, a process requiring Cep290. These studies suggest that Talpid3 and Cep290 play overlapping and distinct roles in ciliary vesicle formation through regulation of centriolar satellite accretion and Rab8a.

Original languageEnglish (US)
Pages (from-to)215-229
Number of pages15
JournalJournal of Cell Biology
Volume204
Issue number2
DOIs
StatePublished - Jan 20 2014

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Cilia
Centrosome
Proteins
Centrioles
Monomeric GTP-Binding Proteins
Protein Transport

ASJC Scopus subject areas

  • Cell Biology

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The CP110-interacting proteins talpid3 and cep290 play overlapping and distinct roles in cilia assembly. / Kobayashi, Tetsuo; Kim, Sehyun; Lin, Yu Chun; Inoue, Takanari; Dynlacht, Brian David.

In: Journal of Cell Biology, Vol. 204, No. 2, 20.01.2014, p. 215-229.

Research output: Contribution to journalArticle

Kobayashi, Tetsuo ; Kim, Sehyun ; Lin, Yu Chun ; Inoue, Takanari ; Dynlacht, Brian David. / The CP110-interacting proteins talpid3 and cep290 play overlapping and distinct roles in cilia assembly. In: Journal of Cell Biology. 2014 ; Vol. 204, No. 2. pp. 215-229.
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