The conversion of [4-14C]-cortisol to [14C]-11β-hydroxyestradiol in a patient with metastatic breast carcinoma-cortisol metabolism and breast cancer

Frank M. Ganis, Ross H. Lowe, Martin H. Morris, Claude J. Migeon, Geary L. Stonesifer

Research output: Contribution to journalArticle

Abstract

The metabolism of cortisol (llβ,17α,21-trihydroxy-4-pregnene-3,20-dione) in an ovariectomized, adrenalectomized patient with breast cancer was investigated. Following administration of [4-14C]-cortisol to the subject, urine samples were collected. The steroid conjugates were extracted from the urine and hydrolyzed, and the free steroids removed by solvent extraction. The estrogen fraction of the urine was separated by sodium hydroxide extraction and purified by counter-current distribution. The extracted estrogen fraction was analyzed by thin-layer chromatography and gas chromatography-mass spectrometry. Gas chromatography-mass spectrometry (GC-MS) of the trimethylsilyl-ethers of the steroids served to identify several radioactive metabolites of [4-14C]-cortisol. One of the urinary metabolites identified was 11β hydroxyestradiol [1,3,5(10)-estratrien-3,11β,17ξ-triol]. The clinical implication of the conversion of an adrenocortical steroid to an estrogenic steroid is discussed.

Original languageEnglish (US)
Pages (from-to)543-549
Number of pages7
JournalJournal of Steroid Biochemistry
Volume5
Issue number6
DOIs
StatePublished - Oct 1974

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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