TY - JOUR
T1 - The conversion of [4-14C]-cortisol to [14C]-11β-hydroxyestradiol in a patient with metastatic breast carcinoma-cortisol metabolism and breast cancer
AU - Ganis, Frank M.
AU - Lowe, Ross H.
AU - Morris, Martin H.
AU - Migeon, Claude J.
AU - Stonesifer, Geary L.
PY - 1974/10
Y1 - 1974/10
N2 - The metabolism of cortisol (llβ,17α,21-trihydroxy-4-pregnene-3,20-dione) in an ovariectomized, adrenalectomized patient with breast cancer was investigated. Following administration of [4-14C]-cortisol to the subject, urine samples were collected. The steroid conjugates were extracted from the urine and hydrolyzed, and the free steroids removed by solvent extraction. The estrogen fraction of the urine was separated by sodium hydroxide extraction and purified by counter-current distribution. The extracted estrogen fraction was analyzed by thin-layer chromatography and gas chromatography-mass spectrometry. Gas chromatography-mass spectrometry (GC-MS) of the trimethylsilyl-ethers of the steroids served to identify several radioactive metabolites of [4-14C]-cortisol. One of the urinary metabolites identified was 11β hydroxyestradiol [1,3,5(10)-estratrien-3,11β,17ξ-triol]. The clinical implication of the conversion of an adrenocortical steroid to an estrogenic steroid is discussed.
AB - The metabolism of cortisol (llβ,17α,21-trihydroxy-4-pregnene-3,20-dione) in an ovariectomized, adrenalectomized patient with breast cancer was investigated. Following administration of [4-14C]-cortisol to the subject, urine samples were collected. The steroid conjugates were extracted from the urine and hydrolyzed, and the free steroids removed by solvent extraction. The estrogen fraction of the urine was separated by sodium hydroxide extraction and purified by counter-current distribution. The extracted estrogen fraction was analyzed by thin-layer chromatography and gas chromatography-mass spectrometry. Gas chromatography-mass spectrometry (GC-MS) of the trimethylsilyl-ethers of the steroids served to identify several radioactive metabolites of [4-14C]-cortisol. One of the urinary metabolites identified was 11β hydroxyestradiol [1,3,5(10)-estratrien-3,11β,17ξ-triol]. The clinical implication of the conversion of an adrenocortical steroid to an estrogenic steroid is discussed.
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U2 - 10.1016/0022-4731(74)90102-2
DO - 10.1016/0022-4731(74)90102-2
M3 - Article
C2 - 4427450
AN - SCOPUS:0016139057
SN - 0022-4731
VL - 5
SP - 543
EP - 549
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 6
ER -