The Construction of nifH-gfp Expression Vector and Its Expression in Enterobacter gergoviae 57-7

Wei Wang, Yue Mei Dong, Qian Li An, Xiang Lin Liu, Jiu Di Li

Research output: Contribution to journalArticlepeer-review

Abstract

A nifH-gfp expression vector pMGFP2 was constructed by fusing the 725 bp PCR amplified triple mutated green fluorescent protein (GFP) gene (gfpS65T, V68L, S72A) fragment to the nifH promoter and its start codon which was from Klebsiella pneumoniae (Schröeter) Trevisan M5a1. A kanamycin cassette was inserted into Pst I site of pMGFP2, obtaining the expressing vector pMGFP2.1 which can be used for the studying of nifH-gfp expression in Enterobacter gergoviae 57-7. It was then transformed into E. gergoviae 57-7 and die effects of NHi+4 and oxygen on the expression of nifH-gfp in E. gergaviae 57-7 were studied.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalActa Botanica Sinica
Volume42
Issue number2
StatePublished - Feb 2000
Externally publishedYes

Keywords

  • Green fluorescent protein
  • nifH promoter
  • nifH-gfp expressing vector

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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