The conserved transcriptional regulator CdnL is required for metabolic homeostasis and morphogenesis in Caulobacter

Selamawit Abi Woldemeskel, Allison K. Daitch, Laura Alvarez, Gaël Panis, Rilee Zeinert, Diego Gonzalez, Erika Smith, Justine Collier, Peter Chien, Felipe Cava, Patrick H. Viollier, Erin D. Goley

Research output: Contribution to journalArticle

Abstract

Bacterial growth and division require regulated synthesis of the macromolecules used to expand and replicate components of the cell. Transcription of housekeeping genes required for metabolic homeostasis and cell proliferation is guided by the sigma factor σ70. The conserved CarD-like transcriptional regulator, CdnL, associates with promoter regions where σ70 localizes and stabilizes the open promoter complex. However, the contributions of CdnL to metabolic homeostasis and bacterial physiology are not well understood. Here, we show that Caulobacter crescentus cells lacking CdnL have severe morphological and growth defects. Specifically, ΔcdnL cells grow slowly in both rich and defined media, and are wider, more curved, and have shorter stalks than WT cells. These defects arise from transcriptional downregulation of most major classes of biosynthetic genes, leading to significant decreases in the levels of critical metabolites, including pyruvate, α-ketoglutarate, ATP, NAD+, UDP-N-acetyl-glucosamine, lipid II, and purine and pyrimidine precursors. Notably, we find that ΔcdnL cells are glutamate auxotrophs, and ΔcdnL is synthetic lethal with other genetic perturbations that limit glutamate synthesis and lipid II production. Our findings implicate CdnL as a direct and indirect regulator of genes required for metabolic homeostasis that impacts morphogenesis through availability of lipid II and other metabolites.

Original languageEnglish (US)
Article numbere1008591
JournalPLoS genetics
Volume16
Issue number1
DOIs
StatePublished - Jan 1 2020

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

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    Woldemeskel, S. A., Daitch, A. K., Alvarez, L., Panis, G., Zeinert, R., Gonzalez, D., Smith, E., Collier, J., Chien, P., Cava, F., Viollier, P. H., & Goley, E. D. (2020). The conserved transcriptional regulator CdnL is required for metabolic homeostasis and morphogenesis in Caulobacter. PLoS genetics, 16(1), [e1008591]. https://doi.org/10.1371/journal.pgen.1008591