The consensus coding sequences of human breast and colorectal cancers

Tobias Sjöblom, Siân Jones, Laura Delong Wood, D. Williams Parsons, Jimmy Lin, Thomas D. Barber, Diana Mandelker, Rebecca J. Leary, Janine Ptak, Natalie Silliman, Steve Szabo, Phillip Buckhaults, Christopher Farrell, Paul Meeh, Sanford D. Markowitz, Joseph Willis, Dawn Dawson, James K V Willson, Adi F. Gazdar, James Hartigan & 9 others Leo Wu, Changsheng Liu, Giovanni Parmigiani, Ben Ho Park, Kurtis E. Bachman, Nickolas Papadopoulos, Bert Vogelstein, Kenneth W Kinzler, Victor E Velculescu

Research output: Contribution to journalArticle

Abstract

The elucidation of the human genome sequence has made it possible to identify genetic alterations in cancers in unprecedented detail. To begin a systematic analysis of such alterations, we determined the sequence of well-annotated human protein-coding genes in two common tumor types. Analysis of 13,023 genes in 11 breast and 11 colorectal cancers revealed that individual tumors accumulate an average of ∼90 mutant genes but that only a subset of these contribute to the neoplastic process. Using stringent criteria to delineate this subset, we identified 189 genes (average of 11 per tumor) that were mutated at significant frequency. The vast majority of these genes were not known to be genetically altered in tumors and are predicted to affect a wide range of cellular functions, including transcription, adhesion, and invasion. These data define the genetic landscape of two human cancer types, provide new targets for diagnostic and therapeutic intervention, and open fertile avenues for basic research in tumor biology.

Original languageEnglish (US)
Pages (from-to)268-274
Number of pages7
JournalScience
Volume314
Issue number5797
DOIs
StatePublished - Oct 13 2006

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Consensus Sequence
Colorectal Neoplasms
Breast Neoplasms
Neoplasms
Genes
Neoplastic Processes
Human Genome
Breast
Research
Proteins

ASJC Scopus subject areas

  • General

Cite this

The consensus coding sequences of human breast and colorectal cancers. / Sjöblom, Tobias; Jones, Siân; Wood, Laura Delong; Parsons, D. Williams; Lin, Jimmy; Barber, Thomas D.; Mandelker, Diana; Leary, Rebecca J.; Ptak, Janine; Silliman, Natalie; Szabo, Steve; Buckhaults, Phillip; Farrell, Christopher; Meeh, Paul; Markowitz, Sanford D.; Willis, Joseph; Dawson, Dawn; Willson, James K V; Gazdar, Adi F.; Hartigan, James; Wu, Leo; Liu, Changsheng; Parmigiani, Giovanni; Park, Ben Ho; Bachman, Kurtis E.; Papadopoulos, Nickolas; Vogelstein, Bert; Kinzler, Kenneth W; Velculescu, Victor E.

In: Science, Vol. 314, No. 5797, 13.10.2006, p. 268-274.

Research output: Contribution to journalArticle

Sjöblom, T, Jones, S, Wood, LD, Parsons, DW, Lin, J, Barber, TD, Mandelker, D, Leary, RJ, Ptak, J, Silliman, N, Szabo, S, Buckhaults, P, Farrell, C, Meeh, P, Markowitz, SD, Willis, J, Dawson, D, Willson, JKV, Gazdar, AF, Hartigan, J, Wu, L, Liu, C, Parmigiani, G, Park, BH, Bachman, KE, Papadopoulos, N, Vogelstein, B, Kinzler, KW & Velculescu, VE 2006, 'The consensus coding sequences of human breast and colorectal cancers', Science, vol. 314, no. 5797, pp. 268-274. https://doi.org/10.1126/science.1133427
Sjöblom T, Jones S, Wood LD, Parsons DW, Lin J, Barber TD et al. The consensus coding sequences of human breast and colorectal cancers. Science. 2006 Oct 13;314(5797):268-274. https://doi.org/10.1126/science.1133427
Sjöblom, Tobias ; Jones, Siân ; Wood, Laura Delong ; Parsons, D. Williams ; Lin, Jimmy ; Barber, Thomas D. ; Mandelker, Diana ; Leary, Rebecca J. ; Ptak, Janine ; Silliman, Natalie ; Szabo, Steve ; Buckhaults, Phillip ; Farrell, Christopher ; Meeh, Paul ; Markowitz, Sanford D. ; Willis, Joseph ; Dawson, Dawn ; Willson, James K V ; Gazdar, Adi F. ; Hartigan, James ; Wu, Leo ; Liu, Changsheng ; Parmigiani, Giovanni ; Park, Ben Ho ; Bachman, Kurtis E. ; Papadopoulos, Nickolas ; Vogelstein, Bert ; Kinzler, Kenneth W ; Velculescu, Victor E. / The consensus coding sequences of human breast and colorectal cancers. In: Science. 2006 ; Vol. 314, No. 5797. pp. 268-274.
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