Abstract
This study investigated the effects of sodium selenite (Se) and of vitamin E (d-α-tochopherol) on the deposition of type I collagen by human LX-2 stellate cells. The cultured cells were treated with or without Se or vitamin E and with or without transforming growth factor β1 (TGFβ1). The combination of Se and vitamin E, but not either alone, protected against hepatic fibrosis by decreasing TGFβ1-mediated collagen secretion and accumulation by the stellate cells. This protective effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen. Effects of Se and vitamin E in decreasing α1(I) collagen mRNA and increasing apoptosis of stellate cells indicate decreased formation of collagen, while decreases in transglutaminase 2, which catalyze cross-linking of collagen, lead to decreased stability of the secreted collagen. Effects of Se and vitamin E on reducing tissue inhibitor metalloproteinase 1 (TIMP-1) are associated with increased degradation. The combination of Se and vitamin E decreased lipid peroxidation, while Se alone increased the activity of the antioxidant enzyme thioredoxin reductase. In conclusion, the combination of Se and vitamin E protected against TGFβ1-mediated hepatic fibrosis by decreasing TGFβ1-mediated type I collagen accumulation by stellate cells. This effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 82-94 |
| Number of pages | 13 |
| Journal | Biological Trace Element Research |
| Volume | 140 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 2011 |
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Keywords
- Apoptosis
- Fibrosis
- Selenium
- TGFbeta1
- Vitamin E
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Inorganic Chemistry
- Biochemistry, medical
- Endocrinology, Diabetes and Metabolism
Cite this
The combination of selenium and vitamin e inhibits type i collagen formation in cultured hepatic stellate cells. / Mezey, Esteban; Liu, Xiaopu; Potter, James John.
In: Biological Trace Element Research, Vol. 140, No. 1, 04.2011, p. 82-94.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The combination of selenium and vitamin e inhibits type i collagen formation in cultured hepatic stellate cells
AU - Mezey, Esteban
AU - Liu, Xiaopu
AU - Potter, James John
PY - 2011/4
Y1 - 2011/4
N2 - This study investigated the effects of sodium selenite (Se) and of vitamin E (d-α-tochopherol) on the deposition of type I collagen by human LX-2 stellate cells. The cultured cells were treated with or without Se or vitamin E and with or without transforming growth factor β1 (TGFβ1). The combination of Se and vitamin E, but not either alone, protected against hepatic fibrosis by decreasing TGFβ1-mediated collagen secretion and accumulation by the stellate cells. This protective effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen. Effects of Se and vitamin E in decreasing α1(I) collagen mRNA and increasing apoptosis of stellate cells indicate decreased formation of collagen, while decreases in transglutaminase 2, which catalyze cross-linking of collagen, lead to decreased stability of the secreted collagen. Effects of Se and vitamin E on reducing tissue inhibitor metalloproteinase 1 (TIMP-1) are associated with increased degradation. The combination of Se and vitamin E decreased lipid peroxidation, while Se alone increased the activity of the antioxidant enzyme thioredoxin reductase. In conclusion, the combination of Se and vitamin E protected against TGFβ1-mediated hepatic fibrosis by decreasing TGFβ1-mediated type I collagen accumulation by stellate cells. This effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen.
AB - This study investigated the effects of sodium selenite (Se) and of vitamin E (d-α-tochopherol) on the deposition of type I collagen by human LX-2 stellate cells. The cultured cells were treated with or without Se or vitamin E and with or without transforming growth factor β1 (TGFβ1). The combination of Se and vitamin E, but not either alone, protected against hepatic fibrosis by decreasing TGFβ1-mediated collagen secretion and accumulation by the stellate cells. This protective effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen. Effects of Se and vitamin E in decreasing α1(I) collagen mRNA and increasing apoptosis of stellate cells indicate decreased formation of collagen, while decreases in transglutaminase 2, which catalyze cross-linking of collagen, lead to decreased stability of the secreted collagen. Effects of Se and vitamin E on reducing tissue inhibitor metalloproteinase 1 (TIMP-1) are associated with increased degradation. The combination of Se and vitamin E decreased lipid peroxidation, while Se alone increased the activity of the antioxidant enzyme thioredoxin reductase. In conclusion, the combination of Se and vitamin E protected against TGFβ1-mediated hepatic fibrosis by decreasing TGFβ1-mediated type I collagen accumulation by stellate cells. This effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen.
KW - Apoptosis
KW - Fibrosis
KW - Selenium
KW - TGFbeta1
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=79952487405&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952487405&partnerID=8YFLogxK
U2 - 10.1007/s12011-010-8672-7
DO - 10.1007/s12011-010-8672-7
M3 - Article
C2 - 20336493
AN - SCOPUS:79952487405
VL - 140
SP - 82
EP - 94
JO - Biological Trace Element Research
JF - Biological Trace Element Research
SN - 0163-4984
IS - 1
ER -