The colorectal microRNAome

Jordan M. Cummins, Yiping He, Rebecca J. Leary, Ray Pagliarini, Luis A. Diaz, Tobias Sjoblom, Omer Barad, Zvi Bentwich, Anna E. Szafranska, Emmanuel Labourier, Christopher K. Raymond, Brian S. Roberts, Hartmut Juhl, Kenneth W. Kinzler, Bert Vogelstein, Victor E. Velculescu

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues.

Original languageEnglish (US)
Pages (from-to)3687-3692
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number10
DOIs
StatePublished - Mar 7 2006

Keywords

  • Colorectal cancer
  • Dicer
  • MicroRNA

ASJC Scopus subject areas

  • General

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