The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis

G. S. Hayward, M. G. Smith

Research output: Contribution to journalArticle

Abstract

We have developed a method for separating the single-stranded DNA fragments from denatured bacteriophage T5 DNA and measuring their molecular weights. This method involves electrophoresis through agarose gels and gives superior resolution to that obtained by sedimentation analysis. With wild-type T5+ DNA, the electrophoretic separations reveal five "major" fragments which have molecular weights of 37.0, 14.5, 13.9, 5.1 and 3.8 million. DNA from the heatstable mutant T5st(O) contains only four major fragments with molecular weights of 35.3, 17.2, 14.5 and 3.8 million. In addition, there are at least 40 discrete "minor" fragments which occur in much smaller amounts and the molecular weights of which range from 0.16 to 28 million. Most of the minor fragments found in T5+ DNA also occur in T5st(O) DNA. We envisage that the major fragments are delineated by "primary" interruptions present in every duplex molecule in the population and that superimposed on this basic structure are "secondary" interruptions which occur only at low frequency. Although these secondary interruptions do not occur at identical positions in all molecules, they give a reproducible pattern of minor fragments for the population as a whole.

Original languageEnglish (US)
Pages (from-to)383-388,IN7-IN8,389-395
JournalJournal of molecular biology
Volume63
Issue number3
DOIs
StatePublished - Feb 14 1972
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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