The chorionic gonadotropin α-subunit gene is on human chromosome 18 in JEG cells

J. W. Hardin, M. E. Riser, J. M. Trent, P. O. Kohler

Research output: Contribution to journalArticlepeer-review

Abstract

The gene for the α subunit of human chorionic gonadotropin (hCG) has been tentatively assigned to human chromosome 18. This localization was accomplished through the use of Southern blot analysis. A full-length cDNA probe for the hCG α subunit and DNA isolated from a series of somatic hybrids between mouse and human cells were utilized to make this assignment. In addition, in situ hybridization with normal human peripheral blood lymphocytes as a source of human chromosomes and with the same cDNA probe confirmed this result. The presence of human chromosome 18 was required for the detection of DNA fragments characteristic of the α-hCG gene. These results are consistent with our previous observation that human chromosomes 10 and 18 are required for the production of hCG in cultured cells.

Original languageEnglish (US)
Pages (from-to)6282-6285
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number20 I
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • General

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