The characterization of la expression during sindbis virus encephalitis in normal and athymic nude mice

William R. Tyor, Guido Stoll, Diane Griffin

Research output: Contribution to journalArticle

Abstract

To characterize the expression of la systemically and locally on mononuclear cells during acute viral encephalitis, weanling mice were inoculated intracerebrally with Sindbis virus (SV), an alphavirus. Peripheral blood mononuclear cells, splenocytes and perivascular inflammatory cells in frozen brain sections were examined immunocytochemically for the presence of la. la expression increased in the spleen, blood and brain during SV encephalitis. The majority of the cells in the central nervous system (CNS) expressing la were perivascular mononuclear cells but la was also found on stellate parenchymal cells. Using one micrometer cryopreserved serial sections we identified these parenchymal cells as macrophages and microglia but not astrocytes. We also identified rare la-positive cells resembling endothelial cells. Frozen brain sections of SV-infected T cell-deficient nu/nu mice were also examined for la expression. The number and percentage of la-positive cells in perivascular inflammatory cells were markedly decreased compared to normal mice and la-positive stellate parenchymal cells were less numerous. This suggests that immunocompetent T cells are necessary for “normal” infiltration of inflammatory cells and for la expression in the CNS during SV encephalitis.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalJournal of Neuropathology and Experimental Neurology
Volume49
Issue number1
StatePublished - 1990

Fingerprint

Sindbis Virus
Encephalitis
Nude Mice
Frozen Sections
Brain
Central Nervous System
Viral Encephalitis
Alphavirus
T-Lymphocytes
Microglia
Astrocytes
Blood Cells
Spleen
Endothelial Cells

Keywords

  • Encephalitis
  • La
  • Microglia
  • Mononuclear cells
  • Sindbis virus

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

The characterization of la expression during sindbis virus encephalitis in normal and athymic nude mice. / Tyor, William R.; Stoll, Guido; Griffin, Diane.

In: Journal of Neuropathology and Experimental Neurology, Vol. 49, No. 1, 1990, p. 21-30.

Research output: Contribution to journalArticle

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