The CD28/B7 Pathway Costimulates the Response of Primary Murine T Cells to Superantigens as Well as to Conventional Antigens

Joel N Blankson, Stephen S. Morse

Research output: Contribution to journalArticle

Abstract

While the CD28/B7 pathway has been shown to play an important costimulatory role in the response of T cells to conventional antigens, its role in superantigen-mediated T cell activation has not been as clear. In this report, we used CD4+ T cells from ovalbumin-specific αβ-TCR-transgenic mice (DO11.10) to compare the ability of this pathway to costimulate the response of primary T cells to conventional antigens and superantigens. We show that either the addition of anti-CD28 monoclonal antibody or presentation with a B7 transfected cell line enhances the proliferative and interleukin-2 secretion response of DO11.10 CD4+ cells to both the conventional antigen, ovalbumin peptide (OVA 323-339), and the superantigen SEB. This implies that CD28-mediated costimulation plays a role in the response of murine T cells to superantigens as well as to conventional antigens.

Original languageEnglish (US)
Pages (from-to)306-312
Number of pages7
JournalCellular Immunology
Volume157
Issue number1
DOIs
StatePublished - Aug 1994
Externally publishedYes

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Superantigens
T-Lymphocytes
Antigens
Ovalbumin
Transgenic Mice
Interleukin-2
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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The CD28/B7 Pathway Costimulates the Response of Primary Murine T Cells to Superantigens as Well as to Conventional Antigens. / Blankson, Joel N; Morse, Stephen S.

In: Cellular Immunology, Vol. 157, No. 1, 08.1994, p. 306-312.

Research output: Contribution to journalArticle

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