The cause of superstoichiometric CA2+ uptake and H+ ejection in L1210 mouse ascites tumor mitochondria

Baltazar Reynafarje; Albert L. Lehninger

doi:10.1016/S0006-291X(74)80388-8

The cause of superstoichiometric CA2+ uptake and H+ ejection in L1210 mouse ascites tumor mitochondria

Baltazar Reynafarje, Albert L. Lehninger

School of Medicine

Research output: Contribution to journal › Article

Abstract

Mitochondria from L1210 mouse leukemia cells show superstoichiometric → H⁺/2e^- ejection and → Ca²⁺/2e^- uptake ratios in the absence of phosphate. Kinetic analysis of Ca²⁺-induced respiratory jumps shows a very rapid, early burst of H⁺ ejection and Ca²⁺ uptake dependent on energy-coupled electron transport but with extraordinarily high stoichiometry (→H⁺/2e^- > 20; →Ca²⁺/2e^- > 10), followed by a slower phase with normal stoichiometry (→H⁺/2e^-=4-5; →Ca²⁺/2e^-=4.0). The early burst of H⁺ ejection, which has a higher K_M for Ca²⁺ than respiratory stimulation, accounts for the superstoichiometry phenomenon; it requires energization by state 4 respiration.

Original language	English (US)
Pages (from-to)	286-292
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	57
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(74)80388-8
State	Published - Mar 15 1974

Fingerprint

Leukemia L1210

Mitochondria

Electron Transport

Ascites

Stoichiometry

Tumors

Respiration

Phosphates

Neoplasms

Kinetics

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Reynafarje, B., & Lehninger, A. L. (1974). The cause of superstoichiometric CA2+ uptake and H+ ejection in L1210 mouse ascites tumor mitochondria. Biochemical and Biophysical Research Communications, 57(1), 286-292. https://doi.org/10.1016/S0006-291X(74)80388-8

The cause of superstoichiometric CA2+ uptake and H+ ejection in L1210 mouse ascites tumor mitochondria. / Reynafarje, Baltazar; Lehninger, Albert L.

In: Biochemical and Biophysical Research Communications, Vol. 57, No. 1, 15.03.1974, p. 286-292.

Research output: Contribution to journal › Article

Reynafarje, B & Lehninger, AL 1974, 'The cause of superstoichiometric CA2+ uptake and H+ ejection in L1210 mouse ascites tumor mitochondria', Biochemical and Biophysical Research Communications, vol. 57, no. 1, pp. 286-292. https://doi.org/10.1016/S0006-291X(74)80388-8

Reynafarje B, Lehninger AL. The cause of superstoichiometric CA2+ uptake and H+ ejection in L1210 mouse ascites tumor mitochondria. Biochemical and Biophysical Research Communications. 1974 Mar 15;57(1):286-292. https://doi.org/10.1016/S0006-291X(74)80388-8

Reynafarje, Baltazar ; Lehninger, Albert L. / The cause of superstoichiometric CA2+ uptake and H+ ejection in L1210 mouse ascites tumor mitochondria. In: Biochemical and Biophysical Research Communications. 1974 ; Vol. 57, No. 1. pp. 286-292.

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10.1016/S0006-291X(74)80388-8