The carnegie protein trap library: A versatile tool for drosophila developmental studies

Michael Buszczak, Shelley Paterno, Daniel Lighthouse, Julia Bachman, Jamie Planck, Stephenie Owen, Andrew D. Skora, Todd G. Nystul, Benjamin Ohlstein, Anna Allen, James E. Wilhelm, Terence D. Murphy, Robert W. Levis, Erika Matunis, Nahathai Srivali, Roger A. Hoskins, Allan C. Spradling

Research output: Contribution to journalArticle

Abstract

Metazoan physiology depends on intricate patterns of gene expression that remain poorly known. Using transposon mutagenesis in Drosophila, we constructed a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, to facilitate gene expression mapping at single-cell resolution. By sequencing the genomic insertion sites, determining splicing patterns downstream of the enhanced green fluorescent protein (EGFP) exon, and analyzing expression patterns in the ovary and salivary gland, we found that 600-900 different genes are trapped in our collection. A core set of 244 lines trapped different identifiable protein isoforms, while insertions likely to act as GFP-enhancer traps were found in 256 additional genes. At least 8 novel genes were also identified. Our results demonstrate that the Carnegie collection will be useful as a discovery tool in diverse areas of cell and developmental biology and suggest new strategies for greatly increasing the coverage of the Drosophila proteome with protein trap insertions.

Original languageEnglish (US)
Pages (from-to)1505-1531
Number of pages27
JournalGenetics
Volume175
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Libraries
Drosophila
Genes
Gene Expression
Developmental Biology
Proteins
Chromosome Mapping
Proteome
Salivary Glands
Mutagenesis
Cell Biology
Ovary
Exons
Protein Isoforms
enhanced green fluorescent protein

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Buszczak, M., Paterno, S., Lighthouse, D., Bachman, J., Planck, J., Owen, S., ... Spradling, A. C. (2007). The carnegie protein trap library: A versatile tool for drosophila developmental studies. Genetics, 175(3), 1505-1531. https://doi.org/10.1534/genetics.106.065961

The carnegie protein trap library : A versatile tool for drosophila developmental studies. / Buszczak, Michael; Paterno, Shelley; Lighthouse, Daniel; Bachman, Julia; Planck, Jamie; Owen, Stephenie; Skora, Andrew D.; Nystul, Todd G.; Ohlstein, Benjamin; Allen, Anna; Wilhelm, James E.; Murphy, Terence D.; Levis, Robert W.; Matunis, Erika; Srivali, Nahathai; Hoskins, Roger A.; Spradling, Allan C.

In: Genetics, Vol. 175, No. 3, 03.2007, p. 1505-1531.

Research output: Contribution to journalArticle

Buszczak, M, Paterno, S, Lighthouse, D, Bachman, J, Planck, J, Owen, S, Skora, AD, Nystul, TG, Ohlstein, B, Allen, A, Wilhelm, JE, Murphy, TD, Levis, RW, Matunis, E, Srivali, N, Hoskins, RA & Spradling, AC 2007, 'The carnegie protein trap library: A versatile tool for drosophila developmental studies', Genetics, vol. 175, no. 3, pp. 1505-1531. https://doi.org/10.1534/genetics.106.065961
Buszczak M, Paterno S, Lighthouse D, Bachman J, Planck J, Owen S et al. The carnegie protein trap library: A versatile tool for drosophila developmental studies. Genetics. 2007 Mar;175(3):1505-1531. https://doi.org/10.1534/genetics.106.065961
Buszczak, Michael ; Paterno, Shelley ; Lighthouse, Daniel ; Bachman, Julia ; Planck, Jamie ; Owen, Stephenie ; Skora, Andrew D. ; Nystul, Todd G. ; Ohlstein, Benjamin ; Allen, Anna ; Wilhelm, James E. ; Murphy, Terence D. ; Levis, Robert W. ; Matunis, Erika ; Srivali, Nahathai ; Hoskins, Roger A. ; Spradling, Allan C. / The carnegie protein trap library : A versatile tool for drosophila developmental studies. In: Genetics. 2007 ; Vol. 175, No. 3. pp. 1505-1531.
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