The canonical Notch pathway effector RBP-J regulates neuronal plasticity and expression of GABA transporters in hippocampal networks

Shuxi Liu, Yue Wang, Paul F Worley, Mark P. Mattson, Nicholas Gaiano

Research output: Contribution to journalArticle

Abstract

Activation of the Notch pathway in neurons is essential for learning and memory in various species from invertebrates to mammals. However, it remains unclear how Notch signaling regulates neuronal plasticity, and whether the transcriptional regulator and canonical pathway effector RBP-J plays a role. Here, we report that conditional disruption of RBP-J in the postnatal hippocampus leads to defects in long-term potentiation, long-term depression, and in learning and memory. Using gene expression profiling and chromatin immunoprecipitation, we identified two GABA transporters, GAT2 and BGT1, as putative Notch/RBP-J pathway targets, which may function downstream of RBP-J to limit the accumulation of GABA in the Schaffer collateral pathway. Our results reveal an essential role for canonical Notch/RBP-J signaling in hippocampal synaptic plasticity and suggest that role, at least in part, is mediated by the regulation of GABAergic signaling.

Original languageEnglish (US)
Pages (from-to)670-678
Number of pages9
JournalHippocampus
Volume25
Issue number5
DOIs
StatePublished - May 1 2015

Fingerprint

GABA Plasma Membrane Transport Proteins
Neuronal Plasticity
Hippocampus
Learning
Long-Term Potentiation
Chromatin Immunoprecipitation
Gene Expression Profiling
Invertebrates
gamma-Aminobutyric Acid
Mammals
Depression
Neurons

Keywords

  • Canonical notch signaling
  • GABA transporter
  • Learning and memory
  • Neuronal plasticity
  • RBP-J

ASJC Scopus subject areas

  • Cognitive Neuroscience

Cite this

The canonical Notch pathway effector RBP-J regulates neuronal plasticity and expression of GABA transporters in hippocampal networks. / Liu, Shuxi; Wang, Yue; Worley, Paul F; Mattson, Mark P.; Gaiano, Nicholas.

In: Hippocampus, Vol. 25, No. 5, 01.05.2015, p. 670-678.

Research output: Contribution to journalArticle

Liu, Shuxi ; Wang, Yue ; Worley, Paul F ; Mattson, Mark P. ; Gaiano, Nicholas. / The canonical Notch pathway effector RBP-J regulates neuronal plasticity and expression of GABA transporters in hippocampal networks. In: Hippocampus. 2015 ; Vol. 25, No. 5. pp. 670-678.
@article{7ab888eefa734799b622d3095b8e543e,
title = "The canonical Notch pathway effector RBP-J regulates neuronal plasticity and expression of GABA transporters in hippocampal networks",
abstract = "Activation of the Notch pathway in neurons is essential for learning and memory in various species from invertebrates to mammals. However, it remains unclear how Notch signaling regulates neuronal plasticity, and whether the transcriptional regulator and canonical pathway effector RBP-J plays a role. Here, we report that conditional disruption of RBP-J in the postnatal hippocampus leads to defects in long-term potentiation, long-term depression, and in learning and memory. Using gene expression profiling and chromatin immunoprecipitation, we identified two GABA transporters, GAT2 and BGT1, as putative Notch/RBP-J pathway targets, which may function downstream of RBP-J to limit the accumulation of GABA in the Schaffer collateral pathway. Our results reveal an essential role for canonical Notch/RBP-J signaling in hippocampal synaptic plasticity and suggest that role, at least in part, is mediated by the regulation of GABAergic signaling.",
keywords = "Canonical notch signaling, GABA transporter, Learning and memory, Neuronal plasticity, RBP-J",
author = "Shuxi Liu and Yue Wang and Worley, {Paul F} and Mattson, {Mark P.} and Nicholas Gaiano",
year = "2015",
month = "5",
day = "1",
doi = "10.1002/hipo.22402",
language = "English (US)",
volume = "25",
pages = "670--678",
journal = "Hippocampus",
issn = "1050-9631",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - The canonical Notch pathway effector RBP-J regulates neuronal plasticity and expression of GABA transporters in hippocampal networks

AU - Liu, Shuxi

AU - Wang, Yue

AU - Worley, Paul F

AU - Mattson, Mark P.

AU - Gaiano, Nicholas

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Activation of the Notch pathway in neurons is essential for learning and memory in various species from invertebrates to mammals. However, it remains unclear how Notch signaling regulates neuronal plasticity, and whether the transcriptional regulator and canonical pathway effector RBP-J plays a role. Here, we report that conditional disruption of RBP-J in the postnatal hippocampus leads to defects in long-term potentiation, long-term depression, and in learning and memory. Using gene expression profiling and chromatin immunoprecipitation, we identified two GABA transporters, GAT2 and BGT1, as putative Notch/RBP-J pathway targets, which may function downstream of RBP-J to limit the accumulation of GABA in the Schaffer collateral pathway. Our results reveal an essential role for canonical Notch/RBP-J signaling in hippocampal synaptic plasticity and suggest that role, at least in part, is mediated by the regulation of GABAergic signaling.

AB - Activation of the Notch pathway in neurons is essential for learning and memory in various species from invertebrates to mammals. However, it remains unclear how Notch signaling regulates neuronal plasticity, and whether the transcriptional regulator and canonical pathway effector RBP-J plays a role. Here, we report that conditional disruption of RBP-J in the postnatal hippocampus leads to defects in long-term potentiation, long-term depression, and in learning and memory. Using gene expression profiling and chromatin immunoprecipitation, we identified two GABA transporters, GAT2 and BGT1, as putative Notch/RBP-J pathway targets, which may function downstream of RBP-J to limit the accumulation of GABA in the Schaffer collateral pathway. Our results reveal an essential role for canonical Notch/RBP-J signaling in hippocampal synaptic plasticity and suggest that role, at least in part, is mediated by the regulation of GABAergic signaling.

KW - Canonical notch signaling

KW - GABA transporter

KW - Learning and memory

KW - Neuronal plasticity

KW - RBP-J

UR - http://www.scopus.com/inward/record.url?scp=84928311014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928311014&partnerID=8YFLogxK

U2 - 10.1002/hipo.22402

DO - 10.1002/hipo.22402

M3 - Article

C2 - 25515406

AN - SCOPUS:84928311014

VL - 25

SP - 670

EP - 678

JO - Hippocampus

JF - Hippocampus

SN - 1050-9631

IS - 5

ER -