The calcineurin-dynamin 1 complex as a calcium sensor for synaptic vesicle endocytosis

Michael M. Lai, Jenny J. Hong, Alicia M. Ruggiero, Patrick E. Burnett, Vladimir I. Slepnev, Pietro De Camilli, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

Exocytosis of synaptic vesicles is calcium-dependent, with synaptotagmin serving as the calcium sensor. Endocytosis of synaptic vesicles has also been postulated as a calcium-dependent process; however, an endocytic calcium sensor has not been found. We now report a physical association between the calcium-dependent phosphatase calcineurin and dynamin 1, a component of the synaptic endocytic machinery. The calcineurin-dynamin 1 interaction is calcium-dependent, with an EC50 for calcium in the range of 0.1-0.4 μM. Disruption of the calcineurin-dynamin 1 interaction inhibits clathrin- mediated endocytosis. Thus, the calcium-dependent formation of the calcineurin-dynamin 1 complex, delivered to the other endocytic coat proteins, provides a calcium-sensing mechanism that facilitates endocytosis.

Original languageEnglish (US)
Pages (from-to)25963-25966
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number37
DOIs
StatePublished - Sep 10 1999

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Lai, M. M., Hong, J. J., Ruggiero, A. M., Burnett, P. E., Slepnev, V. I., De Camilli, P., & Snyder, S. H. (1999). The calcineurin-dynamin 1 complex as a calcium sensor for synaptic vesicle endocytosis. Journal of Biological Chemistry, 274(37), 25963-25966. https://doi.org/10.1074/jbc.274.37.25963