Abstract
Exocytosis of synaptic vesicles is calcium-dependent, with synaptotagmin serving as the calcium sensor. Endocytosis of synaptic vesicles has also been postulated as a calcium-dependent process; however, an endocytic calcium sensor has not been found. We now report a physical association between the calcium-dependent phosphatase calcineurin and dynamin 1, a component of the synaptic endocytic machinery. The calcineurin-dynamin 1 interaction is calcium-dependent, with an EC50 for calcium in the range of 0.1-0.4 μM. Disruption of the calcineurin-dynamin 1 interaction inhibits clathrin- mediated endocytosis. Thus, the calcium-dependent formation of the calcineurin-dynamin 1 complex, delivered to the other endocytic coat proteins, provides a calcium-sensing mechanism that facilitates endocytosis.
Original language | English (US) |
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Pages (from-to) | 25963-25966 |
Number of pages | 4 |
Journal | Journal of Biological Chemistry |
Volume | 274 |
Issue number | 37 |
DOIs | |
State | Published - Sep 10 1999 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology