The C. elegans Myt1 ortholog is required for the proper timing of oocyte maturation

Anna E. Burrows, Bonnielin Swenor, Mary E. Kosinski, Christopher T. Richie, Penny L. Sadler, Jill M. Schumacher, Andy Golden

Research output: Contribution to journalArticle

Abstract

Maturation promoting factor (MPF), a complex of cyclin-dependent kinase 1 and cyclin B, drives oocyte maturation in all animals. Mechanisms to block MPF activation in developing oocytes must exist to prevent precocious cell cycle progression prior to oocyte maturation and fertilization. This study sought to determine the developmental consequences of precociously activating MPF in oocytes prior to fertilization. Whereas depletion of Myt1 in Xenopus oocytes causes nuclear envelope breakdown in vitro, we found that depletion of the Myt1 ortholog WEE-1.3 in C. elegans hermaphrodites causes precocious oocyte maturation in vivo. Although such oocytes are ovulated, they are fertilization incompetent. We have also observed novel phenotypes in these precociously maturing oocytes, such as chromosome coalescence, aberrant meiotic spindle organization, and the expression of a meiosis II post-fertilization marker. Furthermore, co-depletion studies of CDK-1 and WEE-1.3 demonstrate that WEE-1.3 is dispensable in the absence of CDK-1, suggesting that CDK-1 is a major target of WEE-1.3 in C. elegans oocytes.

Original languageEnglish (US)
Pages (from-to)697-709
Number of pages13
JournalDevelopment
Volume133
Issue number4
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Oocytes
Maturation-Promoting Factor
Fertilization
CDC2 Protein Kinase
Cyclin B
Spindle Apparatus
Nuclear Envelope
Meiosis
Xenopus
Cell Cycle
Chromosomes
Phenotype

Keywords

  • Cdk1
  • Meiotic maturation
  • Myt1
  • Oocyte maturation
  • Wee1

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Burrows, A. E., Swenor, B., Kosinski, M. E., Richie, C. T., Sadler, P. L., Schumacher, J. M., & Golden, A. (2006). The C. elegans Myt1 ortholog is required for the proper timing of oocyte maturation. Development, 133(4), 697-709. https://doi.org/10.1242/dev.02241

The C. elegans Myt1 ortholog is required for the proper timing of oocyte maturation. / Burrows, Anna E.; Swenor, Bonnielin; Kosinski, Mary E.; Richie, Christopher T.; Sadler, Penny L.; Schumacher, Jill M.; Golden, Andy.

In: Development, Vol. 133, No. 4, 02.2006, p. 697-709.

Research output: Contribution to journalArticle

Burrows, AE, Swenor, B, Kosinski, ME, Richie, CT, Sadler, PL, Schumacher, JM & Golden, A 2006, 'The C. elegans Myt1 ortholog is required for the proper timing of oocyte maturation', Development, vol. 133, no. 4, pp. 697-709. https://doi.org/10.1242/dev.02241
Burrows AE, Swenor B, Kosinski ME, Richie CT, Sadler PL, Schumacher JM et al. The C. elegans Myt1 ortholog is required for the proper timing of oocyte maturation. Development. 2006 Feb;133(4):697-709. https://doi.org/10.1242/dev.02241
Burrows, Anna E. ; Swenor, Bonnielin ; Kosinski, Mary E. ; Richie, Christopher T. ; Sadler, Penny L. ; Schumacher, Jill M. ; Golden, Andy. / The C. elegans Myt1 ortholog is required for the proper timing of oocyte maturation. In: Development. 2006 ; Vol. 133, No. 4. pp. 697-709.
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