The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation

Mike Boxem; Chiawei W. Tsai; Yi Zhang; R. Mako Saito; Jun O. Liu

doi:10.1016/j.febslet.2004.08.077

The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation

Mike Boxem, Chiawei W. Tsai, Yi Zhang, R. Mako Saito, Jun O. Liu

School of Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We have investigated the physiological function of type 2 methionine aminopeptidases (MetAP2) using Caenorhabditis elegans as a model system. A homolog of human MetAP2 was found in the C. elegans genome, which we termed MAP-2. MAP-2 protein displayed methionine aminopeptidase activity and was sensitive to inhibition by fumagillin. Downregulation of map-2 expression by RNAi led to sterility, resulting from a defect in germ cell proliferation. These observations suggest that MAP-2 is essential for germ cell development in C. elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner.

Original language	English (US)
Pages (from-to)	245-250
Number of pages	6
Journal	FEBS Letters
Volume	576
Issue number	1-2
DOIs	https://doi.org/10.1016/j.febslet.2004.08.077
State	Published - Oct 8 2004

Keywords

Germline development
MAP, methionine aminopeptidase
MetAP, methionine aminopeptidase
Methionine aminopeptidase
ORF, open reading frame
P.I., propidium iodide
RNAi, RNA interference
dsRNA, double-stranded RNA
map-1
map-2

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2004.08.077

Cite this

@article{e3fe62c506b749e78851e88a08f4e477,

title = "The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation",

abstract = "We have investigated the physiological function of type 2 methionine aminopeptidases (MetAP2) using Caenorhabditis elegans as a model system. A homolog of human MetAP2 was found in the C. elegans genome, which we termed MAP-2. MAP-2 protein displayed methionine aminopeptidase activity and was sensitive to inhibition by fumagillin. Downregulation of map-2 expression by RNAi led to sterility, resulting from a defect in germ cell proliferation. These observations suggest that MAP-2 is essential for germ cell development in C. elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner.",

keywords = "Germline development, MAP, methionine aminopeptidase, MetAP, methionine aminopeptidase, Methionine aminopeptidase, ORF, open reading frame, P.I., propidium iodide, RNAi, RNA interference, dsRNA, double-stranded RNA, map-1, map-2",

author = "Mike Boxem and Tsai, {Chiawei W.} and Yi Zhang and Saito, {R. Mako} and Liu, {Jun O.}",

note = "Funding Information: We are grateful to Sander van den Heuvel and Marc Vidal for their generous support during different phases of this work and to Dr. Yie-Hwa Chang for the anti-human MetAP2 antibody. We thank David Greenstein for the lim-7::GFP reporters strain and Tim Schedl for the glp-1 gain-of-function mutant strain. We also thank Geraldine Seydoux for critical comments on the manuscript. Research support was provided in part by an NIH grant (CA078743) to J.O.L. M.B. was supported by a Boehringer Ingelheim Fonds PhD grant and a Leukemia Research Foundation postdoctoral fellowship.",

year = "2004",

month = oct,

day = "8",

doi = "10.1016/j.febslet.2004.08.077",

language = "English (US)",

volume = "576",

pages = "245--250",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation

AU - Boxem, Mike

AU - Tsai, Chiawei W.

AU - Zhang, Yi

AU - Saito, R. Mako

AU - Liu, Jun O.

N1 - Funding Information: We are grateful to Sander van den Heuvel and Marc Vidal for their generous support during different phases of this work and to Dr. Yie-Hwa Chang for the anti-human MetAP2 antibody. We thank David Greenstein for the lim-7::GFP reporters strain and Tim Schedl for the glp-1 gain-of-function mutant strain. We also thank Geraldine Seydoux for critical comments on the manuscript. Research support was provided in part by an NIH grant (CA078743) to J.O.L. M.B. was supported by a Boehringer Ingelheim Fonds PhD grant and a Leukemia Research Foundation postdoctoral fellowship.

PY - 2004/10/8

Y1 - 2004/10/8

N2 - We have investigated the physiological function of type 2 methionine aminopeptidases (MetAP2) using Caenorhabditis elegans as a model system. A homolog of human MetAP2 was found in the C. elegans genome, which we termed MAP-2. MAP-2 protein displayed methionine aminopeptidase activity and was sensitive to inhibition by fumagillin. Downregulation of map-2 expression by RNAi led to sterility, resulting from a defect in germ cell proliferation. These observations suggest that MAP-2 is essential for germ cell development in C. elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner.

AB - We have investigated the physiological function of type 2 methionine aminopeptidases (MetAP2) using Caenorhabditis elegans as a model system. A homolog of human MetAP2 was found in the C. elegans genome, which we termed MAP-2. MAP-2 protein displayed methionine aminopeptidase activity and was sensitive to inhibition by fumagillin. Downregulation of map-2 expression by RNAi led to sterility, resulting from a defect in germ cell proliferation. These observations suggest that MAP-2 is essential for germ cell development in C. elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner.

KW - Germline development

KW - MAP, methionine aminopeptidase

KW - MetAP, methionine aminopeptidase

KW - Methionine aminopeptidase

KW - ORF, open reading frame

KW - P.I., propidium iodide

KW - RNAi, RNA interference

KW - dsRNA, double-stranded RNA

KW - map-1

KW - map-2

UR - http://www.scopus.com/inward/record.url?scp=4944221796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4944221796&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2004.08.077

DO - 10.1016/j.febslet.2004.08.077

M3 - Article

C2 - 15474045

AN - SCOPUS:4944221796

SN - 0014-5793

VL - 576

SP - 245

EP - 250

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this