The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation

Mike Boxem, Chiawei W. Tsai, Yi Zhang, R. Mako Saito, Jun O. Liu

Research output: Contribution to journalArticle

Abstract

We have investigated the physiological function of type 2 methionine aminopeptidases (MetAP2) using Caenorhabditis elegans as a model system. A homolog of human MetAP2 was found in the C. elegans genome, which we termed MAP-2. MAP-2 protein displayed methionine aminopeptidase activity and was sensitive to inhibition by fumagillin. Downregulation of map-2 expression by RNAi led to sterility, resulting from a defect in germ cell proliferation. These observations suggest that MAP-2 is essential for germ cell development in C. elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalFEBS Letters
Volume576
Issue number1-2
DOIs
StatePublished - Oct 8 2004

Keywords

  • Germline development
  • MAP, methionine aminopeptidase
  • MetAP, methionine aminopeptidase
  • Methionine aminopeptidase
  • ORF, open reading frame
  • P.I., propidium iodide
  • RNAi, RNA interference
  • dsRNA, double-stranded RNA
  • map-1
  • map-2

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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