The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules

Ekaterina Voronina, Geraldine Seydoux

Research output: Contribution to journalArticlepeer-review

Abstract

C. elegans P granules are conserved cytoplasmic ribonucleoprotein complexes that are unique to the germline and essential for fertility. During most of germline development, P granules are perinuclear and associate with clusters of nuclear pores. In an RNAi screen against nucleoporins, we have identified a specific nucleoporin essential for P granule integrity and function. The C. elegans homolog of vertebrate Nup98 (CeNup98) is enriched in P granules and associates with the translationally repressed, P granule-enriched mRNA nos-2 (nanos homolog). Loss of CeNup98 causes P granules to disperse in the cytoplasm and to release nos-2 mRNA. Embryos depleted for CeNup98 express a nos-2 3′UTR reporter prematurely. In the mouse, Nup98 immunoprecipitates with the germ granule component MVH. Our findings suggest that, in germ cells, the function of Nup98 extends beyond transport at the nuclear pore to include mRNA regulation in the cytoplasm.

Original languageEnglish (US)
Pages (from-to)1441-1450
Number of pages10
JournalDevelopment
Volume137
Issue number9
DOIs
StatePublished - May 1 2010

Keywords

  • C. Elegans
  • Germline
  • Nucleoporin
  • Nup98
  • P granule
  • Translational control

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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