The breathless FGF receptor homolog, a downstream target of Drosophila C/EBP in the developmental control of cell migration

A. M. Murphy, T. Lee, C. M. Andrews, B. Z. Shilo, D. J. Montell

Research output: Contribution to journalArticle

Abstract

To investigate the molecular mechanisms responsible for the temporal and spatial control of cell movements during development, we have been studying the migration of a small group of follicle cells, called the border cells, in the Drosophila ovary. Timely initiation of border cell migration requires the product of the slow border cells (slbo) locus, which encodes the Drosophila homolog of the transcription factor C/EBP. Here we report evidence that one target of C/EBP in the control of border cell migration is the FGF receptor homolog encoded by the breathless (btl) locus, btl expression in the ovary was border cell-specific, beginning just prior to the migration, and this expression was reduced in slbo mutants, btl mutations dominantly enhanced the border cell migration defects found in weak slbo alleles. Furthermore, C/EBP-independent btl expression was able to rescue the migration defects of hypomorphic slbo alleles. Purified Drosophila C/EBP bound eight sites in the btl 5' flanking region by DNAse I footprinting. Taken together these results suggest that btl is a key, direct target for C/EBP in the regulation of border cell migration.

Original languageEnglish (US)
Pages (from-to)2255-2263
Number of pages9
JournalDevelopment
Volume121
Issue number8
StatePublished - 1995

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Fibroblast Growth Factor Receptors
Drosophila
Cell Movement
Ovary
Alleles
5' Flanking Region
Transcription Factors
Mutation

Keywords

  • Border cells
  • Breathless
  • C/EBP
  • Cell migration
  • Drosophila
  • FGF receptor

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Murphy, A. M., Lee, T., Andrews, C. M., Shilo, B. Z., & Montell, D. J. (1995). The breathless FGF receptor homolog, a downstream target of Drosophila C/EBP in the developmental control of cell migration. Development, 121(8), 2255-2263.

The breathless FGF receptor homolog, a downstream target of Drosophila C/EBP in the developmental control of cell migration. / Murphy, A. M.; Lee, T.; Andrews, C. M.; Shilo, B. Z.; Montell, D. J.

In: Development, Vol. 121, No. 8, 1995, p. 2255-2263.

Research output: Contribution to journalArticle

Murphy, AM, Lee, T, Andrews, CM, Shilo, BZ & Montell, DJ 1995, 'The breathless FGF receptor homolog, a downstream target of Drosophila C/EBP in the developmental control of cell migration', Development, vol. 121, no. 8, pp. 2255-2263.
Murphy, A. M. ; Lee, T. ; Andrews, C. M. ; Shilo, B. Z. ; Montell, D. J. / The breathless FGF receptor homolog, a downstream target of Drosophila C/EBP in the developmental control of cell migration. In: Development. 1995 ; Vol. 121, No. 8. pp. 2255-2263.
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