Abstract
Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. Here, we describe a novel method for detecting proteins that contain nitrosothiols. In this three-step procedure, nitrosylated cysteines are converted to biotinylated cysteines. Biotinylated proteins can then be detected by immunoblotting or can be purified by avidin-affinity chromatography. We include examples of the detection of S-nitrosylated proteins in brain lysates after in vitro S-nitrosylation, as well as the detection of endogenous S-nitrosothiols in selected neuronal proteins.
Original language | English (US) |
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Pages (from-to) | PL1 |
Journal | Science's STKE : signal transduction knowledge environment |
Volume | 2001 |
Issue number | 86 |
DOIs | |
State | Published - Jun 12 2001 |
ASJC Scopus subject areas
- General Medicine