The Binding of the Circumsporozoite Protein to Cell Surface Heparan Sulfate Proteoglycans is Required for Plasmodium Sporozoite Attachment to Target Cells

Consuelo Pinzon-Ortiz, Jennifer Friedman, Jeffrey Esko, Photini Sinnis

Research output: Contribution to journalArticle

Abstract

The major surface protein of malaria sporozoites, the circumsporozoite protein, binds to heparan sulfate proteoglycans on the surface of hepatocytes. It has been proposed that this binding event is responsible for the rapid and specific localization of sporozoites to the liver after their injection into the skin by an infected anopheline mosquito. Previous in vitro studies performed under static conditions have failed to demonstrate a significant role for heparan sulfate proteoglycans during sporozoite invasion of cells. We performed sporozoite attachment and invasion assays under more dynamic conditions and found a dramatic decrease in sporozoite attachment to cells in the presence of heparin. In contrast to its effect on attachment, heparin does not appear to have an effect on sporozoite invasion of cells. When substituted heparins were used as competitive inhibitors of sporozoite attachment, we found that sulfation of the glycosaminoglycan chains at both the N- and O-positions was important for sporozoite adhesion to cells. We conclude that the binding of the circumsporozoite protein to hepatic heparan sulfate proteoglycans is likely to function during sporozoite attachment in the liver and that this adhesion event depends on the sulfated glycosaminoglycan chains of the proteoglycans.

Original languageEnglish (US)
Pages (from-to)26784-26791
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number29
DOIs
StatePublished - Jul 20 2001
Externally publishedYes

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Sporozoites
Heparan Sulfate Proteoglycans
Plasmodium
Heparin
Carrier Proteins
A73025
Liver
Adhesion
Proteins
Proteoglycans
Glycosaminoglycans
Assays
Skin
Membrane Proteins
Culicidae
Cell Adhesion
Malaria
Hepatocytes
Injections

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Binding of the Circumsporozoite Protein to Cell Surface Heparan Sulfate Proteoglycans is Required for Plasmodium Sporozoite Attachment to Target Cells. / Pinzon-Ortiz, Consuelo; Friedman, Jennifer; Esko, Jeffrey; Sinnis, Photini.

In: Journal of Biological Chemistry, Vol. 276, No. 29, 20.07.2001, p. 26784-26791.

Research output: Contribution to journalArticle

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