The bacteriophage λ O and P protein initiators promote the replication of single-stranded DNA

Jonathan H. Lebowitz, Roger Mcmacken

Research output: Contribution to journalArticle

Abstract

A soluble enzyme system that specifically initiates λdy plasmid DNA replication at a bacteriophage λ replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and øX174 to a duplex form. This chain initiation on single-stranded templates is novel in that it is absolutely dependent on the λ O and P protein chromosomal initiators and on several Escherichia coli proteins that are known to function in the replication of the λ chromosome in vivo, including the host dnaB, dnaG (primase), dnaJ and dnaK replication proteins. Strand initiation occurs at multiple sites following an O and P protein-dependent pre-priming step in which the DNA is converted into an activated nucleoprotein complex containing the bacterial dnaB protein. We propose a scheme for the initiation of DNA synthesis on single-stranded templates in this enzyme system that may be relevant to strand initiation events that occur during replication of phage λ in vivo.

Original languageEnglish (US)
Pages (from-to)3069-3088
Number of pages20
JournalNucleic acids research
Volume12
Issue number7
DOIs
StatePublished - Apr 11 1984

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'The bacteriophage λ O and P protein initiators promote the replication of single-stranded DNA'. Together they form a unique fingerprint.

  • Cite this