TY - JOUR
T1 - The bacteriophage λ O and P protein initiators promote the replication of single-stranded DNA
AU - Lebowitz, Jonathan H.
AU - Mcmacken, Roger
N1 - Funding Information:
ACKNOWLEDGMENTS We are especially indebted to Drs. Maciej Zylicz and Costa Georgopoulos for donation of purified dnaJ and dnaK proteins and for their assistance in the development of the dnaJ complementation assay. We also thank Dr. Arthur Kornberg and Dr. Charles McHenry for their gifts of antibodies and DNA polymerase III holoenzyme, respectively. This research was supported by grants from the American Cancer Society (NP-363) and from the U.S. Public Health Service (GM32253 and ES/CAO3131).
PY - 1984/4/11
Y1 - 1984/4/11
N2 - A soluble enzyme system that specifically initiates λdy plasmid DNA replication at a bacteriophage λ replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and øX174 to a duplex form. This chain initiation on single-stranded templates is novel in that it is absolutely dependent on the λ O and P protein chromosomal initiators and on several Escherichia coli proteins that are known to function in the replication of the λ chromosome in vivo, including the host dnaB, dnaG (primase), dnaJ and dnaK replication proteins. Strand initiation occurs at multiple sites following an O and P protein-dependent pre-priming step in which the DNA is converted into an activated nucleoprotein complex containing the bacterial dnaB protein. We propose a scheme for the initiation of DNA synthesis on single-stranded templates in this enzyme system that may be relevant to strand initiation events that occur during replication of phage λ in vivo.
AB - A soluble enzyme system that specifically initiates λdy plasmid DNA replication at a bacteriophage λ replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and øX174 to a duplex form. This chain initiation on single-stranded templates is novel in that it is absolutely dependent on the λ O and P protein chromosomal initiators and on several Escherichia coli proteins that are known to function in the replication of the λ chromosome in vivo, including the host dnaB, dnaG (primase), dnaJ and dnaK replication proteins. Strand initiation occurs at multiple sites following an O and P protein-dependent pre-priming step in which the DNA is converted into an activated nucleoprotein complex containing the bacterial dnaB protein. We propose a scheme for the initiation of DNA synthesis on single-stranded templates in this enzyme system that may be relevant to strand initiation events that occur during replication of phage λ in vivo.
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U2 - 10.1093/nar/12.7.3069
DO - 10.1093/nar/12.7.3069
M3 - Article
C2 - 6326050
AN - SCOPUS:0021760294
SN - 0305-1048
VL - 12
SP - 3069
EP - 3088
JO - Nucleic acids research
JF - Nucleic acids research
IS - 7
ER -