THE AUTOANTIBODY NATURE OF THE THYROIDITIS ANTIBODY AND THE ROLE OF THYROGLOBULIN IN THE REACTION

Ernest Witebsky; Noel R. Rose; Sidney Shulman

doi:10.1016/S0140-6736(58)91737-9

THE AUTOANTIBODY NATURE OF THE THYROIDITIS ANTIBODY AND THE ROLE OF THYROGLOBULIN IN THE REACTION

Ernest Witebsky, Noel R. Rose, Sidney Shulman

School of Medicine

Research output: Contribution to journal › Article › peer-review

Original language	English (US)
Pages (from-to)	808-810
Number of pages	3
Journal	The Lancet
Volume	271
Issue number	7025
DOIs	https://doi.org/10.1016/S0140-6736(58)91737-9
State	Published - Apr 19 1958

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@article{2e86157cadc6434cb8d38039211aa2e1,

title = "THE AUTOANTIBODY NATURE OF THE THYROIDITIS ANTIBODY AND THE ROLE OF THYROGLOBULIN IN THE REACTION",

author = "Ernest Witebsky and Rose, {Noel R.} and Sidney Shulman",

note = "Funding Information: III, IV, and V. The only other ultracentrifugal component available in purified form is peak V, the thyralbumin fraction, obtained by precipitation between 2-50 and 3-00 M ammonium sulphate (Shulman et al. 1957). To measure the antigen content of the various thyroid fractions agglutination-inhibition tests were set up. Serial dilutions of each fraction were prepared and added to aliquots of the Buc. serum diluted to contain about four (2 +) hxmagglutination doses. After an hour at room temperature tanned human red cells coated with the Buc. thyroid extract were added to each tube, and the degree of agglutination was read in the usual way. Table ill shows the highest dilution of each thyroid com- ponent capable of completely inhibiting agglutination. The initial saline extract, as well as the thyroglobulin fraction, can neutralise the reaction of Buc. serum in high dilutions and are almost equally effective. The thyral- bumin fraction and the supernatant from the thyroglobulin precipitation show relatively little activity. These findings suggest that the antigen involved in the autoimmunisation leading to thyroiditis precipitates in the thyroglobulin fraction, and that this antigen can elicit demonstrable circulating antibodies in the same individual. We wish to acknowledge the capable technical assistance of Miss Dolores Floss and Miss Helen Stuerzebecker. The work was supported in part by grant C-2357 from the National Cancer Institute.",

year = "1958",

month = apr,

day = "19",

doi = "10.1016/S0140-6736(58)91737-9",

language = "English (US)",

volume = "271",

pages = "808--810",

journal = "The Lancet",

issn = "0140-6736",

publisher = "Elsevier Limited",

number = "7025",

}

TY - JOUR

T1 - THE AUTOANTIBODY NATURE OF THE THYROIDITIS ANTIBODY AND THE ROLE OF THYROGLOBULIN IN THE REACTION

AU - Witebsky, Ernest

AU - Rose, Noel R.

AU - Shulman, Sidney

N1 - Funding Information: III, IV, and V. The only other ultracentrifugal component available in purified form is peak V, the thyralbumin fraction, obtained by precipitation between 2-50 and 3-00 M ammonium sulphate (Shulman et al. 1957). To measure the antigen content of the various thyroid fractions agglutination-inhibition tests were set up. Serial dilutions of each fraction were prepared and added to aliquots of the Buc. serum diluted to contain about four (2 +) hxmagglutination doses. After an hour at room temperature tanned human red cells coated with the Buc. thyroid extract were added to each tube, and the degree of agglutination was read in the usual way. Table ill shows the highest dilution of each thyroid com- ponent capable of completely inhibiting agglutination. The initial saline extract, as well as the thyroglobulin fraction, can neutralise the reaction of Buc. serum in high dilutions and are almost equally effective. The thyral- bumin fraction and the supernatant from the thyroglobulin precipitation show relatively little activity. These findings suggest that the antigen involved in the autoimmunisation leading to thyroiditis precipitates in the thyroglobulin fraction, and that this antigen can elicit demonstrable circulating antibodies in the same individual. We wish to acknowledge the capable technical assistance of Miss Dolores Floss and Miss Helen Stuerzebecker. The work was supported in part by grant C-2357 from the National Cancer Institute.

PY - 1958/4/19

Y1 - 1958/4/19

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U2 - 10.1016/S0140-6736(58)91737-9

DO - 10.1016/S0140-6736(58)91737-9

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AN - SCOPUS:49749162573

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SN - 0140-6736

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ER -

THE AUTOANTIBODY NATURE OF THE THYROIDITIS ANTIBODY AND THE ROLE OF THYROGLOBULIN IN THE REACTION

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