The attachment of minicircles to kinetoplast DNA networks during replication

David L. Pérez-Morga, Paul T. Englund

Research output: Contribution to journalArticle

Abstract

Kinetoplast DNA (kDNA), the trypanosomatid mitochondrial DNA, is a network containing several thousand interlocked minicircles. During kDNA synthesis, minicircles dissociate from the network, and after replication their progeny reattach to the network periphery. Using electron microscopy autoradiography, we found that newly synthesized 3H-labeled minicircles, after short labeling periods, are concentrated in two peripheral zones on opposite sides of the network. These must be minicircle attachment sites, adjacent to the two diametrically opposed complexes of replication proteins observed previously. From the pattern of radiolabeling during longer pulses, we reached the unexpected conclusion that minicircle attachment around the entire network periphery may be due to a relative movement of the kinetoplast and the two complexes. The kinetoplast probably rotates between two fixed complexes.

Original languageEnglish (US)
Pages (from-to)703-711
Number of pages9
JournalCell
Volume74
Issue number4
DOIs
StatePublished - Aug 27 1993

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Kinetoplast DNA
Autoradiography
Mitochondrial DNA
Labeling
Electron microscopy
Electron Microscopy
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The attachment of minicircles to kinetoplast DNA networks during replication. / Pérez-Morga, David L.; Englund, Paul T.

In: Cell, Vol. 74, No. 4, 27.08.1993, p. 703-711.

Research output: Contribution to journalArticle

Pérez-Morga, David L. ; Englund, Paul T. / The attachment of minicircles to kinetoplast DNA networks during replication. In: Cell. 1993 ; Vol. 74, No. 4. pp. 703-711.
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