The association of allergic sensitization patterns in early childhood with disease manifestations and immunological reactivity at 10 years of age

Véronique Schulten, April Frazier, Agustin Calatroni, Meyer Kattan, Leonard B. Bacharier, George T. O’Connor, Megan T. Sandel, Robert A Wood, Lisa M. Wheatley, Alkis Togias, Cynthia M. Visness, Amy Dresen, James E. Gern, Alessandro Sette

Research output: Contribution to journalArticle

Abstract

Background: Allergy to German cockroach (CR) is common in urban environments and is an important allergen in children with asthma. Objective: We hypothesize that the evolution of allergic sensitization and clinical disease is associated with distinct patterns of allergen-specific T cell reactivity. To test this hypothesis, a subset of high-risk inner-city children participating in the URECA (Urban Environment and Childhood Asthma) birth cohort were selected to evaluate CR-specific T cell reactivity from three distinct groups based on acquisition of aeroallergen sensitivity from ages 2 to 10: low atopy with minimal to no sensitivity (n = 26), early-onset allergic sensitization (n = 25) and late-onset allergic sensitization (n = 25). Methods: Using pools of previously identified CR-derived T cell epitopes, we characterized the allergen-specific T cell response in these 76 subjects from blood samples obtained at age 10. CR-specific production of IL-5, IFNγ and IL-10 was measured by ELISPOT following two-week in vitro culture with CR extract. Results: T cell responses were significantly higher in the early-onset atopy group compared to low atopy (P = 0.01), and a trend for higher cytokine production in the late onset compared to the low atopy cohort was also observed (P = 0.06). T cell responses were similar between early- and late-onset cohorts. Furthermore, a comparison of T cell reactivity between asthmatic and non-asthmatic individuals revealed significantly higher cytokine production in asthmatics compared to non-asthmatics (P = 0.02) within both the CR-allergic and non-allergic cohorts. Conclusions and clinical relevance: In conclusion, the present study reports that higher T cell reactivity is associated with allergen sensitization and asthma. Interestingly, no significant difference in T cell reactivity was observed in allergic children with early-onset versus late-onset atopy.

Original languageEnglish (US)
Pages (from-to)1087-1094
Number of pages8
JournalClinical and Experimental Allergy
Volume49
Issue number8
DOIs
StatePublished - Jan 1 2019

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Immune System Diseases
Cockroaches
T-Lymphocytes
Allergens
Asthma
Blattellidae
Cytokines
Enzyme-Linked Immunospot Assay
T-Lymphocyte Epitopes
Interleukin-5
Interleukin-10
Hypersensitivity
Parturition

Keywords

  • allergens and epitopes
  • asthma
  • IgE
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

The association of allergic sensitization patterns in early childhood with disease manifestations and immunological reactivity at 10 years of age. / Schulten, Véronique; Frazier, April; Calatroni, Agustin; Kattan, Meyer; Bacharier, Leonard B.; O’Connor, George T.; Sandel, Megan T.; Wood, Robert A; Wheatley, Lisa M.; Togias, Alkis; Visness, Cynthia M.; Dresen, Amy; Gern, James E.; Sette, Alessandro.

In: Clinical and Experimental Allergy, Vol. 49, No. 8, 01.01.2019, p. 1087-1094.

Research output: Contribution to journalArticle

Schulten, V, Frazier, A, Calatroni, A, Kattan, M, Bacharier, LB, O’Connor, GT, Sandel, MT, Wood, RA, Wheatley, LM, Togias, A, Visness, CM, Dresen, A, Gern, JE & Sette, A 2019, 'The association of allergic sensitization patterns in early childhood with disease manifestations and immunological reactivity at 10 years of age', Clinical and Experimental Allergy, vol. 49, no. 8, pp. 1087-1094. https://doi.org/10.1111/cea.13406
Schulten, Véronique ; Frazier, April ; Calatroni, Agustin ; Kattan, Meyer ; Bacharier, Leonard B. ; O’Connor, George T. ; Sandel, Megan T. ; Wood, Robert A ; Wheatley, Lisa M. ; Togias, Alkis ; Visness, Cynthia M. ; Dresen, Amy ; Gern, James E. ; Sette, Alessandro. / The association of allergic sensitization patterns in early childhood with disease manifestations and immunological reactivity at 10 years of age. In: Clinical and Experimental Allergy. 2019 ; Vol. 49, No. 8. pp. 1087-1094.
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abstract = "Background: Allergy to German cockroach (CR) is common in urban environments and is an important allergen in children with asthma. Objective: We hypothesize that the evolution of allergic sensitization and clinical disease is associated with distinct patterns of allergen-specific T cell reactivity. To test this hypothesis, a subset of high-risk inner-city children participating in the URECA (Urban Environment and Childhood Asthma) birth cohort were selected to evaluate CR-specific T cell reactivity from three distinct groups based on acquisition of aeroallergen sensitivity from ages 2 to 10: low atopy with minimal to no sensitivity (n = 26), early-onset allergic sensitization (n = 25) and late-onset allergic sensitization (n = 25). Methods: Using pools of previously identified CR-derived T cell epitopes, we characterized the allergen-specific T cell response in these 76 subjects from blood samples obtained at age 10. CR-specific production of IL-5, IFNγ and IL-10 was measured by ELISPOT following two-week in vitro culture with CR extract. Results: T cell responses were significantly higher in the early-onset atopy group compared to low atopy (P = 0.01), and a trend for higher cytokine production in the late onset compared to the low atopy cohort was also observed (P = 0.06). T cell responses were similar between early- and late-onset cohorts. Furthermore, a comparison of T cell reactivity between asthmatic and non-asthmatic individuals revealed significantly higher cytokine production in asthmatics compared to non-asthmatics (P = 0.02) within both the CR-allergic and non-allergic cohorts. Conclusions and clinical relevance: In conclusion, the present study reports that higher T cell reactivity is associated with allergen sensitization and asthma. Interestingly, no significant difference in T cell reactivity was observed in allergic children with early-onset versus late-onset atopy.",
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AU - Sandel, Megan T.

AU - Wood, Robert A

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